摘要
目的:分析血管内皮生长因子(vascular endothelial growth factor,VEGF),miR-205,Ezrin,Lamin A/C在卵巢癌组织中的表达及意义。方法:采用酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)检测卵巢癌患者和正常女性血清中VEGF的含量;免疫组织化学检测上皮性卵巢癌组织、卵巢良性肿瘤组织和正常卵巢组织中血管内皮细胞生长因子受体-1(vascular endothelial cell growth factor receptor-1,VEGFR-1),VEGFR-2,Ezrin,Lamin A/C蛋白的表达情况和CD31所标记的微血管密度(microvessel density,MVD);采用荧光定量PCR方法检测卵巢上皮性癌组织、卵巢良性肿瘤组织和正常卵巢组织中miR-205,Ezrin和Lamin A/C的相对表达量。结果:VEGF在上皮性卵巢癌患者血清中的表达水平为(116.10±11.94),高于正常女性(40.04±4.97,P<0.05);VEGFR-1和VEGFR-2在卵巢上皮性癌组织中的阳性表达率分别为75.9%和91.4%,高于卵巢良性肿瘤组织和正常卵巢组织中的阳性表达率(P<0.05),VEGFR-1和VEGFR-2在卵巢良性肿瘤组织和正常卵巢组织中的表达差异无统计学意义(P>0.05);MVD在上皮性卵巢癌组织中的计数为(7.56±0.51),高于正常卵巢组织(1.22±0.56,P<0.05),MVD在卵巢良性肿瘤组织和正常卵巢组织中的表达差异无统计学意义(P>0.05);miR-205在上皮性卵巢癌组织中的相对表达量为(0.106±0.035),高于正常卵巢组织中的相对表达量(0.0007±0.0005,P<0.05),miR-205在卵巢良性肿瘤组织中的相对表达量为(0.0002±0.0003),高于正常卵巢癌组织中的相对表达量,但差异无统计学意义(P>0.05);免疫组织化学结果显示:Ezrin和Lamin A/C在上皮性卵巢癌组织中的阳性表达率分别为51.7%和60.3%,低于卵巢良性肿瘤组织和正常卵巢组织中的阳性表达率(P<0.05),Ezrin和Lamin A/C在卵巢良性肿瘤组织和正常卵巢组织中的表达差异无统计学意义(P>0.05);RT-PCR结果显示:Ezrin和Lamin A/C在上皮性卵巢癌组织中的相对表达量低于正常卵巢
Objective: To analyze the expression of vascular endothelial growth factor(VEGF), miR-205, Ezrinand Lamin A/C in ovarian cancer tissues. Methods: The expression of VEGF in the serum of epithelial ovarian cancer and that of healthy volunteers were detected by enzyme-linked immunosorbent assay; the expressions of vascular endothelial growth factor receptor 1(VEGFR-1), VEGFR-2, Ezrin and Lamin A/C were detected by immunohistochemistry and the micro-vessel density(MVD) of CD31 was detected by immunohistochemistry in epithelial ovarian cancer, benign ovarian and normal ovarian specimens; and the expression of miR-205, Ezrin and Lamin A/C were detected by real-time PCR in epithelial ovarian cancer, benign ovarian and normal ovarian specimens. Results: The expression of VEGF in the serum of epithelial ovarian cancer patients(116.10± 11.94) was signii cantly higher than that of healthy volunteers(40.04±4.97, P<0.05). h e positive expression rates of VEGFR-1 and VEGFR-2 in the epithelial ovarian cancer specimens were 75.9% and 91.4% respectively, which were significantly higher than that in the benign ovarian and the normal ovarian specimens(P<0.05). No differences were observed in the positive expression rates of VEGFR-1 and VEGFR-2 between the benign ovarian and the normal ovarian specimens(P>0.05). The average length of MVD in the epithelial ovarian cancer specimens(7.56±0.51), was signii cantly higher than that in the normal ovarian specimens(1.22±0.56, P<0.05) and in the benign ovarian specimens(0.7±0.39, P<0.05). No dif erences were observed in the average length of MVD between the benign ovarian and the normal ovarian specimens(P>0.05). The relative expression level of miR-205 was 0.106±0.035 in the epithelial ovarian cancer specimens, which was significantly higher than that in the normal ovarian specimens(0.0007±0.0005, P<0.05); the relative expression level of miR-205 in the benign ovarian specimens was(0.0002±0.0002), higher than that in the normal ovarian specimens, but with no signii cance(P>0.05). h
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2014年第2期142-150,共9页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(81172469)
中南大学研究生学位论文创新基金(2011ssxt108)~~
