摘要
目的结合不同提取方式建立湿法消解-电感耦合等离子体发射光谱法(inductively coupled plasma optical emission spectrometry,ICP-OES)检测富硒豆类中硒蛋白含量的方法。方法分别以水、0.5 mol/L氯化钠溶液、75%乙醇(V:V)、0.1 mol/L氢氧化钠溶液为溶剂,超声辅助提取富硒豆类中的硒蛋白,硝酸-过氧化氢体系湿法消解样品,采用ICP-OES检测硒蛋白含量。结果以氢氧化钠溶液作提取剂,硒元素分析谱线为196.0 nm,检测效果最佳,线性关系良好,相关系数大于0.99,检出限为0.082 mg/kg,定量限为0.272 mg/kg。硒标准溶液的加标回收率为90.0 %-98.5%,相对标准偏差为3.1%-6.3%,硒代蛋氨酸标准溶液的加标回收率为84.0 %-92.8%,相对标准偏差为0.2%-1.8%。结论该方法前处理提取效果好,湿式消解法消解样品完全,方法的物理化学干扰少,具有良好的准确度和精密度,适用于富硒豆类食品中硒蛋白含量的准确检测。
Objective To establish a method for the determination of selenium protein in Se-enriched beans using wet digestion-inductively coupled plasma optical emission spectrometry(ICP-OES) combined with different extraction methods was proposed.Methods Four extraction solvents:Water,sodium chloride solution(0.5 mol/L),ethanol(75%,V:V) and sodium hydroxide solution(0.1 mol/L) were used to extract selenium protein in Se-enriched beans assisted by ultrasonic,the samples were digested with nitric acid hydrogen peroxide system by wet digestion method,and the selenium protein were determined by ICP-OES.Results Sodium hydroxide solution was used as extractant and the spectral line of selenium was 196.0 nm,the result was the best,the selenium elements had good linear relationships,and the correlation coefficients were greater than 0.99,the limits of detection were 0.082 mg/kg,the limits of quantification were 0.272 mg/kg.The recoveries of selenium standard solution were 90.0%-98.5%,and the relative standard deviations were 3.1%-6.3%.The recoveries of selenomethionine standard solution were84.0%-92.8%,and the relative standard deviations were 0.2%-1.8%.Conclusion This method has the best extraction effect,the samples can be completely digested by wet digestion,the method has less physical and chemical interference and good accuracy and precision,which is suitable for the accurate detection of selenium protein in Se-enriched beans.
作者
贾玮
周媛媛
许牡丹
石琳
莫海珍
张浩
夏曾润
祁蒙
JIA Wei;ZHOU Yuan-Yuan;XU Mu-Dan;SHI Lin;MO Hai-Zhen;ZHANG Hao;XIA Zeng-Run;QI Meng(School of Food and Biological Engineering,Shaanxi University of Science and Technology,Xi'an 710021,China;Shaanxi Research Institute of Agricultural Products Processing Technology,Xi'an 710021,China;School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China;Ankang Research and Development Center for Se-enriched Products,Ankang 725000,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2021年第19期7520-7526,共7页
Journal of Food Safety and Quality
基金
国家重点研发计划项目(2019YFC1606306-2)
中国富硒产业研究院富硒产业全产业链专项研发计划项目(2020FXZX01)
陕西省重点研发计划项目(2021NY-185、2020NY-097)
河南省重点研发计划项目(202102110017)。
关键词
湿法消解
电感耦合等离子体发射光谱法
富硒豆类
硒蛋白
wet digestion
inductively coupled plasma optical emission spectrometry
Se-enriched beans
selenium protein
