摘要
为明确引起山东某猪场猪群暴发临床急性死亡的主要致病菌,本研究采用生化鉴定试验、动物试验,并利用PCR方法扩增分离菌的GroEl基因片段进行菌株鉴定;同时通过药敏试验分析该菌的耐药性。结果表明,该菌为猪丹毒杆菌(命名为SD-196403),能够发酵葡萄糖、果糖和乳糖,不能发酵蔗糖、鼠李糖等。小鼠攻毒试验表明该菌有很强的致病力。药敏试验结果表明该菌对头孢克肟、恩诺沙星、氨苄西林和卡那霉素等敏感,对林可霉素耐药。
To identify the main pathogens causing bacterial infection in a pig farm of Shandong Province,the bacterial morphology observation,Gram stain test,biochemical test,PCR test on the GroEl gene fragment,animal experiment,and drug sensitive test were carried out to identify the isolated strain.The results showed that the isolated strain named as SD-196403 was as pathogenic Erysipelothrix erysipeloides,and capable of fermenting glucose and fructose but not sucrose and rhamnose.The isolated strain had strong pathogenicity.The drug sensitivity test indicated that the isolated strain was sensitive to cefaclor,enrofloxacin,ampicillin and kanamycin,but resistant to lincomycin.
作者
张瑞华
任丹宁
高善颂
窦心怡
张丹萍
李焕发
韩先杰
Zhang Ruihua;Ren Danning;Gao Shansong;Dou Xinyi;Zhang Danping;Li Huanfa;Han Xianjie(College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China)
出处
《山东农业科学》
北大核心
2021年第10期123-127,共5页
Shandong Agricultural Sciences
基金
山东省重大科技创新工程项目(2019JZZY010720)
山东省现代农业产业技术体系生猪创新团队资助项目(SDAIT-08-10)。
关键词
猪丹毒杆菌
GroEl基因
生化鉴定
药敏试验
Erysipelothrix erysipeloides
GroEl gene
Biochemical identification
Drug sensitivity test
