摘要
目的了解绍兴市2017—2019年沙门菌病人分离株的分子分型特征及耐药情况。方法收集245株分离自绍兴市食源性腹泻病例中的沙门菌,进行血清学分型,采用微量肉汤稀释法进行药敏检测,使用脉冲场凝胶电泳(PFGE)对分离株进行分子分型,利用BioNumerics V7.1软件进行聚类分析。结果245株沙门菌可分为60种血清型,优势血清型为鼠伤寒沙门菌(32.24%)、肠炎沙门菌(10.20%)和伦敦沙门菌(6.94%)。225株分离株对25种抗生素存在不同程度的耐药,其中氨苄西林(77.14%)、四环素(73.88%)和链霉素(66.53%)的耐药率较高,且多重耐药率达76.33%。XbaⅠ酶切后的鼠伤寒沙门菌、肠炎沙门菌和伦敦沙门菌分别含48种、11种和12种不同PFGE指纹图谱。结论绍兴市沙门菌血清型种类繁多,PFGE指纹图谱呈多样性,抗生素耐药呈现较集中且主要为氨苄西林-四环素-链霉素(AMP-TET-STR)。
Objective To understand the molecular epidemiology and antimicrobial resistance pattern of clinically isolated Salmonella in Shaoxing city from 2017 to 2019.Methods The 245 Salmonella strains that were isolated from foodborne disease and diarrhea cases in Shaoxing were collected and serotyped.Broth microdilution method was performed to carry out drug resistance testing.Pulsed field gel electrophoresis(PFGE)and BioNumerics V7.1 software were applied to clustering analysis.Results The 245 isolates of Salmonella were composed of sixty serotypes.The predominant serovars were Salmonella typhimurium(32.24%),Salmonella enteritidis(10.20%)and Salmonella london(6.94%).The 225 strains were resistant to 25 antibiotics at different degrees.Among these isolates,higher rates of resistance to ampicillin(77.14%),tetracycline(73.88%)and streptomycin(66.53%)were found and the multiple drug resistance rate reached 76.33%.On the other hand,the predominant serovars were digested with XbaⅠ,which contained 48,11 and 12 different PFGE fingerprints,respectively.Conclusion Salmonella serotypes and PFGE fingerprints were highly diverse.Antibiotic resistance was concentrated in AMP-TET-STR.
作者
张琴超
陈金堃
陈吉铭
何琴芬
ZHANG Qinchao;CHEN Jinkun;CHEN Jiming;HE Qinfen(Shaoxing Center for Disease Control and Prevention,Zhejiang Shaoxing 312071,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2021年第5期542-548,共7页
Chinese Journal of Food Hygiene
基金
浙江省公共卫生应急检测关键技术重点实验室开放基金(2020-02)。
关键词
沙门菌
血清型分型
耐药
脉冲场凝胶电泳
Salmonella
serotype
antimicrobial resistance
pulsed field gel electrophoresis
