摘要
【目的】基于转录组数据鉴定分析叶用莴苣MADS-box基因家族,筛选高温促进叶用莴苣抽薹过程中MADS-box家族特异性表达的基因,以期为叶用莴苣高温诱导抽薹的分子机制鉴定提供理论依据。【方法】从叶用莴苣转录组数据中鉴定获得MADS-box家族转录因子并进行生物信息学分析,利用qRT-PCR技术分析叶用莴苣易抽薹品种‘S39’在高温处理下MADS-box基因的表达模式。【结果】在叶用莴苣转录组中共鉴定到14个MADS-box转录因子,其编码的蛋白质含有212~253个氨基酸,蛋白质大小为24026.50~29380.16 Da,理论等电点范围为5.27~9.44,其中2个为稳定蛋白,12个属于不稳定蛋白,均为亲水性蛋白。亚细胞定位预测结果显示所有蛋白质均定位于细胞核。qRT-PCR结果表明,在高温处理下,LsMADS 32表达量无显著规律;LsMADS16和LsMADS37表达总体呈下降趋势;LsMADS5,LsMADS14,LsMADS15,LsMADS29,LsMADS35,LsMADS39,LsMADS43,LsMADS45,LsMADS48,LsMADS54和LsMADS56表达趋势基本相同,呈先上升后下降趋势,LsMADS15在高温处理7 d后达到峰值,其他基因在高温处理5 d后达到峰值,其中LsMADS 54在高温处理5 d后骤升1000倍,LsMADS 56在高温处理5 d后骤升70倍。【结论】MADS-box家族中的部分成员可能与高温诱导抽薹密切相关,LsMADS 54和LsMADS56是叶用莴苣响应高温诱导后抽薹开花调控网络中的潜在的重要转录因子。
[Objective]In order to provide theory basis for molecular mechanism of high temperature induced bolting in lettuce,MADS-box gene family of lettuce was identified and analyzed based on transcriptome data and MADS-box genes specifically expressed in the process of high temperature induced bolting were screened.[Methods]With the transcriptome data of lettuce collected,transcription factors of MADS-box family were identified and analyzed by bioinformatic while the expression of 14 bolting-related MADS-box family genes in leaves of'S39',which was the earliest bolting variety,was analyzed by qRT-PCR under high temperature treatment at 1 d,3 d,5 d,7 d,9 d,11 d,13 d and 15 d.[Results]14 MADS-box transcription factors were identified in lettuce transcriptome.The proteins encoded by MADS-box transcriptome contained 212-253 amino acids,a protein size of 24026.50-29380.16 Da,and a theoretical isoelectric point range of 5.27-9.44.2 proteins encoded by MADS-box were stable and 12 proteins were unstable.All proteins were hydrophilic proteins.The prediction results of subcellular localization showed that all proteins were localized in the nucleus.As was shown in the qRT-PCR results,under heat treatment conditions,the expression pattern of LsMADS 32 was not significant.The expression of LsMADS16 and LsMADS37 showed a decreasing trend.The expression trends of LsMADS5,LsMADS14,LsMADS15,LsMADS29,LsMADS35,LsMADS39,LsMADS43,LsMADS45,LsMADS54,LsMADS48 and LsMADS56 were basically the same,which increased firstly and then decreased.LsMADS15 reached the peak value on the 7th day of high temperature treatment,and other genes reached the peak value on the 5th day of high temperature treatment,among which LsMADS 54 increased 1000 times and LsMADS 56 increased 70 times on the 5th day of high temperature treatment.[Conclusion]These findings indicate that some MADS-box genes may be closely related to bolting,and LsMADS 54 and LsMADS 56 are potentially important transcription factors in the network of bolting and flowering in lettuce in response
作者
郝新迪
许梦男
董波波
陈丽
刘超杰
范双喜
韩莹琰
HAO Xindi;XU Mengnan;DONG Bobo;CHEN Li;LIU Chaojie;FAN Shuangxi;HAN Yingyan(Beijing Key Laboratory for Agricultural Application and New Technique,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)
出处
《北京农学院学报》
2021年第4期14-19,共6页
Journal of Beijing University of Agriculture
基金
国家自然科学基金项目(31772321)
北京市委组织部青年拔尖人才项目(2018000026833ZK76)
现代农业产业技术体系北京市叶类蔬菜创新团队项目(BAIC07-2021)。
