摘要
目的基于网络药理学探讨紫苏子-杏仁药对治疗支气管哮喘的作用机制。方法基于中药系统药理学数据库和分析平台(TCMSP)收集紫苏子-杏仁药对的有效活性成分及其靶点信息。通过Genecards数据库获取哮喘相关基因,并与经STRING数据库转换的紫苏子-杏仁药对的靶基因进行匹配,得到紫苏子-杏仁药对治疗哮喘的候选作用靶点。利用STRING数据库和Cytoscape软件构建药物-有效活性成分-作用靶点网络和蛋白互作网络图,并利用DAVID数据库对预测靶点进行GO富集分析和KEGG通路富集分析。结果筛选得到紫苏子中有效活性成分14个、杏仁中有效活性成分15个,关键有效活性成分包括雌酮、木犀草素、β-谷固醇等。去重后得到两者共有靶点179个,关键靶点包括TP53、AKT1、JUN、CDKN1A、VEGFA等。GO富集分析结果包括GO-BP(生物过程)、GO-MF(分子功能)和GO-CC(细胞组分)分析。KEGG通路富集分析得到7条与哮喘显著相关通路。结论紫苏子-杏仁药对主要通过PI3K-Akt、TNF、P53等信号通路发挥治疗哮喘作用。
Objective To predict the mechanism of perilla seed-almond pairs(PSAPs)on bronchial asthma using network pharmacology.Methods The active components and target information of PSAPs were collected from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Asthma-related genes were obtained from Genecards database and matched with the target genes of PSAPs transformed by STRING database,and the candidate targets of PSAPs for asthma treatment were achieved.Target network and protein interaction network were constructed using STRING database and Cytoscape software.GO enrichment and KEGG pathway of predicted targets were analyzed using David Database.Results The active components were 14 in perilla seed and 15 in almond,of which estrone,luteolin,andβ-sitosterol were crucial.After removing the duplicates,179 targets were obtained,with key targets including TP53,AKT1,JUN,CDKN1A,VEGFA,etc.GO enrichment analysis showed biological process,molecular function and cell component.KEGG pathway enrichment analysis revealed 7 asthma-associated pathways.Conclusion PSAPs could treat bronchial asthma through PI3K/AKT,TNF and P53 pathways.
作者
黄芳芳
吴锐剑
李晓玲
林浩雯
邱琴
林柏忻
黄宇戈
罗连响
HUANG Fang-fang;WU Rui-jian;LI Xiao-ling;LIN Hao-wen;QIU Qin;LIN Bai-xin;HUANG Yu-ge;LUO Lian-xiang(Graduate School,Guangdong Medical University,Zhanjiang 524023,China;Experimental Animal Center,Guangdong Medical University,Zhanjiang 524023,China;First Clinical College,Guangdong Medical University,Zhanjiang 524023,China;Affiliated Hospital,Guangdong Medical University,Zhanjiang 524023,China;Marine Biomedical Research Institute,Guangdong Medical University,Zhanjiang 524023,China)
出处
《广东医科大学学报》
2021年第5期550-557,共8页
Journal of Guangdong Medical University
基金
广东省基础与应用基础研究基金项目(No.2019 A1515110201)
广东省中医药局中医药科研项目(No.20201180)
湛江市科技计划项目(No.2019A01009)
广东医科大学博士科研启动项目(No.B2019016)。
关键词
紫苏子
杏仁
网络药理学
支气管哮喘
perilla seed
almond
network pharmacology
bronchial asthma
