摘要
2018年8月24日,在3口面积均为8×667 m^(2)、主养草鱼Ctenopharyngodon idellus(1400 kg)混养滤食性鱼类(700 kg)、水源、形状、水深和投饲管理等均一致的池塘中,分别均匀泼洒红糖活化2 h后的EM菌及芽孢杆菌75 g/667m^(2)(泼菌组);先后泼洒过磷酸钙(有效P2O5为500 g/667 m^(2))及同量EM菌和芽孢杆菌(加磷泼菌组);对照组仅泼洒红糖溶剂(质量与前两组相同)。泼菌前1 d和泼菌后10 d采集这3个池塘的水样,测定浮游植物群落和水体氮磷含量的变化,探究施用微生物制剂对精养池中浮游植物群落结构和水质的影响,为提高微生物制剂调水效果提供依据。结果表明:微生物制剂有短期去除水体总氮效果,对水体总磷变化无明显影响,但是,适量补磷,可改善池塘的磷限制,促进微生物制剂对水体的净化效果;施用微生物制剂组水体中绿藻门及藻类种类数增多,生物量增加;群落的个体小型化趋势得到抑制,对Simpson和Shannon-Wiener多样性变化无明显影响。各组主要种分布差异较为明显,试验前后对照组的主要种均属于中-富营养型的蓝藻门,而泼菌组与加磷泼菌组均增加1种对水环境要求更高的绿藻门藻类,分别为单角盘星藻Pediastrum simplex和纤细新月藻Closterium gracile Breb.。
Three 8×667 m^(2) ponds stocked with principal grass carp(Ctenopharyngodon idellus)(1400 kg)and polycultured with filter-feeding fish(700 kg)were sprayed evenly with EM bacteria and Bacillus spp.activated by brown sugar uniformly sprinkled for two h(spill bacteria group)at dose of 75 g/667m^(2);with calcium superphosphate(500 g/667m^(2) of effective P2O5)and the same amount of EM bacteria and Bacillus sp.(phosphate-bacteria group),and only sprinkled with brown sugar solvent(quality and the first two groups)(control group),respectively,on August 24,2018.The changes in phytoplankton community and contents of nitrogen and phosphorus in the water body were determined in the water samples from these three ponds collected one day before and 10 days after administration to explore the effects of application of microbial preparations on structure of phytoplankton communities and water quality in the intensive freshwater fish ponds.The results showed that the probiotics removed the total nitrogen in water in a short time,without significant influence on the change in total phosphorus in the water in a short period of time.However,the appropriate amount of phosphorus supplementation with N/P ratio(N/P)of more than 10 led to promote the purification effect of the microbial preparation on the water.The increase in the total species of chlorophyta and biomass was observed in the group treated with the probiotics,which was mainly contributed by species in Chlorophyta,and the trend of individual miniaturization in the community was inhibited,without significant impact on the diversity indices of Simpson and Shannon-Wiener.In the control group,all the main species before and after the experiment belonged to the medium-eutrophic Cyanobacteria,while in the two groups,one species in Chlorophyta with higher requirements for water environment,Pediastrum simplex and Closterium gracile Breb,was added during the experiment.The findings indicated that the microbial agents have positive effects on phytoplankton community changes in inten
作者
向劲
程小飞
谢敏
李金龙
宋锐
彭治桃
XIANG Jin;CHENG Xiaofei;XIE Min;LI Jinlong;SONG Rui;PENG Zhitao(Hunan Fisheries Science Institute,Changsha 410153,China)
出处
《水产学杂志》
CAS
北大核心
2021年第4期66-72,共7页
Chinese Journal of Fisheries
基金
国家大宗淡水鱼产业技术体系长沙综合试验站(CARS-45-47).
关键词
浮游植物
微生物制剂
氮磷比
生态响应
phytoplankton
microbial agents
nitrogen and phosphorus ratio
ecological response
