摘要
目的探讨过氧化物酶2(Prx2)在单侧输尿管梗阻(UUO)大鼠肾组织中的表达情况。方法将24只雄性SD大鼠随机分假手术组(n=8)和UUO组(n=16)。假手术组术后3 d处死大鼠并获取肾组织,UUO组分别于术后3、7、14、21 d处死大鼠并获取肾组织。采用HE染色评估大鼠肾纤维化模型;免疫组化检测Prx2的表达状况;实时PCR与Western blotting检测Prx2、a-SMA、Vimentin mRNA和蛋白的表达。结果与假手术组比较,UUO组肾间质炎症细胞浸润,肾小管扩张,胶原沉积增加,肾纤维化模型建立成功;UUO组肾组织中Prx2、α-SMA和Vimentin mRNA和蛋白表达水平随时间延长逐渐增高(均P<0.01)。结论Prx2在UUO大鼠肾组织中高表达,呈现时间依赖性,其表达增高可能与肾纤维化程度密切相关。
Objective To investigate the expression of peroxiredoxin 2(Prx2)in renal tissues of unilateral ureteral obstruction(UUO)rats.Methods Twenty-four male SD rats were randomly divided into sham(n=8)and UUO(n=16,rat renal fibrosis model was established)groups.Rats in sham group were sacrificed on day 3 post-surgery,and those in UUO group were sacrificed on days 3,7,14 and 21 post-surgery,respectively,and then,kidney tissues were harvested.HE staining was performed to evaluate the rat renal fibrosis model.The expression of Prx2 was detected through immunohistochemical analysis.The mRNA and protein expressions of Prx2,α-SMA,and Vimentin were detected through real-time PCR and Western blotting.Results Compared with sham group,UUO group exhibited renal interstitial inflammatory cell infiltration,dilated renal tubules,increased collagen deposition,and eventually,renal fibrosis model was established successfully.The mRNA and protein expression levels of Prx2,α-SMA,and Vimentin in UUO group were found to be increased with time(all P<0.01).Conclusion Prx2 expression increased in renal tissues of UUO rats in a time-dependent manner,and the increase in its expression may be closely related to the degree of renal fibrosis.
作者
秦思文
姜红
QIN Siwen;JIANG Hong(Department of Pediatrics,The First Hospital of China Medical University,Shenyang 110001,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2021年第10期940-943,共4页
Journal of China Medical University
关键词
单侧输尿管梗阻
大鼠
肾组织
过氧化物酶2
unilateral ureteral obstruction
rat
renal tissues
peroxiredoxin 2
