摘要
介绍了光学相干层析成像在不断提升其性能方面的一些研究,其中主要包括在成像的分辨率、成像速度、成像探头等基础核心指标方面大幅提升的代表性工作。综述了清华大学物理系近二十多年来在光学相干层析成像研究方面的一系列重要进展,包括取得了纵向分辨率为0.64μm的高分辨率成像、E3.5长时间细胞活体的亚细胞分辨成像,成功研制了速度高达40 MHz的高速扫频激光器,实现了基于光计算的每秒1000万次轴向线扫描的超高速实时光学成像信息处理等。
Significance Optical coherence tomography(OCT)is a depth-resolved biomedical in vivo imaging technique providing cross-sectional and three-dimensional(3D)images of tissue microstructure with a micrometer-scale resolution.In the past few decades,OCT has been employed in various applications,including clinical and material research areas.In this paper,some studies on improving the performance of OCT are introduced.Among them,imaging resolution,imaging speed,and multifunctional integration are the basic core indicators of the imaging system.Recently,research on OCT conducted at the Department of Physics of Tsinghua University has achieved a series of important advances in improving imaging system performance.Progress For high-resolution imaging,we achieved ultrahigh-resolution OCT with a supercontinuum by coupling femtosecond pulses generated from a commercial Ti∶sapphire laser into an air-silica microstructure fiber.The visible supercontinuum from 450 to 700 nm centered at 540 nm was generated.A free-space axial OCT resolution of 0.64μm was achieved.The sensitivity of the OCT system was 108 dB with an incident light power of 3 mW at a sample,only 7 dB below the theoretical limit.For subcellular and long-time in vivo imaging,we developed a novel system of full-field OCT(FF-OCT)for label-free 3D subcellular in vivo imaging of preimplantation mouse embryos.As the sample received much less optical dose than in conventional confocal imaging,the preimplantation mouse embryos were alive even after several days’ live imaging.Various typical preimplantation stages,including zygote,two-cell,four-cell,and blastocyst(at embryonic day 3.5,E3.5 for short),were investigated with a spatial resolution of 0.7μm and imaging rate of 24 frame/s.These are the first in vivo studies with mammalian embryos at the beginning of their embryonic lives for understanding early patterning and polarity.For high-speed imaging,a high-speed swept source is necessary for system setup.Real-time 3 Dhigh-definition OCT imaging requires 1000 x-scan�
作者
薛平
Xue Ping(State Key Laboratory of Low-Dimensional Quantum Physics and Department of Physics,Tsinghua University,Beijing 100084,China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2021年第15期392-402,共11页
Chinese Journal of Lasers
基金
国家自然科学基金(69908004,90508001,61227807,61575108)
科技部国家高技术研究发展计划(2006AA02Z472)
国家重点基础研究发展计划(2009CB929400)。
关键词
成像系统
光学相干层析成像
光纤激光器
光在组织中传播
光数据处理
imaging system
optical coherence tomography
fiber lasers
light propagation in tissues
optical data processing
