摘要
为了研究油藏内源微生物好氧和厌氧激活规律,在亨盖特厌氧微生物操作平台上,对沾3区块油井产出液分别进行好氧和厌氧激活研究。结果表明:油藏内源微生物在好氧和厌氧激活方式下均能被有效激活,激活5 d以后,菌密度超过10^(8)个/mL,但不同激活方式激活的菌群结构、驱油功能菌存在显著差异。在好氧激活下的微生物代谢速率快,开始产乳化剂时间比厌氧激活早;厌氧条件下,加入磷酸盐、硝酸盐,提供电子受体能够显著促进厌氧乳化,乳化指数可达100%。但氧化还原电位的提高,对内源微生物厌氧代谢产酸、产气有一定抑制作用,总酸含量从2.769 g/L降至1.046 g/L,气压从0.156 MPa降至0.060 MPa,CH_(4)含量从31.1%降至5.6%。室内模拟研究揭示的微生物厌氧激活特征为中高温油藏内源微生物驱油厌氧代谢调控提供了重要理论指导。
To study the rules of aerobic and anaerobic activation of endogenous microorganisms in oil reservoir,the aerobic and anaerobic activation of produced fluid from Zhan3 oil well were studied on hungate anaerobic microbial operation platform.Results showed that the oil reservoir endogenous microorganisms could be effectively activated under different activation modes,and the bacterial concentration was more than 10^(8) mL^(-1) after 5 days activation.However,there were significant differences in the structure of microbial community and function bacteria activated by different activation modes.The microbial metabolism rate was fast under aerobic activation,and the emulsifier production time was earlier than that of anaerobic activation.The phosphate and nitrate were added,then the electron acceptor produced under anaerobic conditions could significantly promote anaerobic emulsification,and the emulsification index reached 100%.However,the increase of redox potential had a certain inhibitory effect on the acid and gas production of endogenous microbial anaerobic metabolisms.The total acid content was reduced from 2.769 g/L to 1.046 g/L,the gas pressure was reduced from 0.156 MPa to 0.060 MPa,and the methane content was reduced from 31.1%to 5.6%.The characteristics of microbial anaerobic activation revealed by laboratory simulation provided important theoretical guidance for the regulation of anaerobic metabolism of endogenous microbial flooding in medium and high temperature oil reservoirs.
作者
李金志
冯云
林军章
谭晓明
王静
吴晓玲
LI Jinzhi;FENG Yun;LIN Junzhang;TAN Xiaoming;WANG Jing;WU Xiaoling(Oil and gas development management center, Shengli Oil Field, Sinopec, Dongying 257000, China;Research Institute of Petroleum Engineering, Shengli Oil Field, Sinopec, Dongying 257000, China)
出处
《南京工业大学学报（自然科学版）》
CAS
北大核心
2021年第5期670-676,共7页
Journal of Nanjing Tech University(Natural Science Edition)
基金
国家自然科学基金(51774188)。
关键词
内源微生物
好氧激活
厌氧激活
生物乳化剂
生物气
endogenous microorganisms
aerobic activation
anaerobic activation
bioemulsifier
biogenic gas
