摘要
目的:研究三种动物油脂对乙醇致大鼠胃粘膜损伤的影响。方法:分别以低(500 mg/kg)、高剂量(850 mg/kg)的鹿油、牛油、猪油对大鼠连续灌胃30 d后,除正常组外,其余各组大鼠灌胃给予无水乙醇1.0 mL/只,1 h后麻醉,心脏取血,取胃组织并称重,对大鼠胃粘膜进行形态学观察、组织病理学检查以及急性损伤指标的测定,并检测各组大鼠血清中超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)含量,利用ELISA法测定血清中肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)的含量变化,并用RT-PCR技术检测促红细胞生成素(EPO)和促红细胞生成素受体(EPOR)的mRNA表达水平。结果:与模型组相比,各给药组大鼠在各时期体重均无明显变化,鹿油预处理组大鼠胃重量/体质量极显著降低(P<0.001),胃粘膜表面较少出血带且出血带较细,细胞排列紧密有序,炎性浸润减少,胃粘膜充血面积、损伤积分指数均极显著降低(P<0.001),能极显著提高GSH-Px活力、SOD活性(P<0.01)并显著降低MDA水平(P<0.05),进而提高机体抗氧化能力来保护胃黏膜,鹿油高度显著降低血清中炎症因子TNF-α和IL-6水平,能够调控乙醇对胃粘膜造成损伤所产生的炎症因子,使EPO和EPOR的mRNA表达降低;牛油预处理组大鼠胃粘膜表面多条细长出血带,颜色略深,细胞排列较为有序,胃粘膜充血面积、损伤积分指数降低,可极显著提高SOD活力(P<0.001)、明显降低MDA水平来提升抗氧化能力保护胃黏膜;猪油预处理组大鼠胃粘膜表面有多条较宽出血带,颜色较深,对急性胃粘膜损伤无明显影响。结论:三种动物油脂对急性胃粘膜损伤的保护作用依次是:鹿油>牛油>猪油,鹿油对大鼠急性胃粘膜损伤具有明显保护作用,牛油保护作用较弱,猪油则无明显保护作用。
Objective:To study the effects of three kinds of animal fats on gastric mucosa damage induced by ethanol in rats.Methods:Low(500 mg/kg)and high doses(850 mg/kg)of deer oil,butter and lard were given to rats by gavage for 30 days.Except for the normal group,rats in the other groups were given 1.0 mL of absolute ethanol per rat.One hour later,blood was taken from anesthesia,and gastric tissue was taken.Morphological observation,histopathological examination and the determination of acute injury indexes were performed on the gastric mucosa of rats.Detect the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in serum of rats in each group.The ELISA method was used to determine the serum levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6),and RT-PCR was used to detect erythropoietin(EPO)and erythropoietin receptor(EPOR)mRNA expression level.Results:Compared with the model group,the weight of rats in each administration group did not change significantly in each period.The stomach weight/body mass of the rats in the deer oil pretreatment group was extremely significantly reduced(P<0.001),and the gastric mucosal surface had fewer bleeding bands and thinner bleeding bands.The cells were arranged tightly and orderly,the inflammatory infiltration was reduced,the gastric mucosal congestion area and the injury score index were extremely significantly redu(P<0.001)ced,which could significantly increase the activity of GSH-Px,SOD activity(P<0.01)and significantly reduced the level of MDA(P<0.05),thereby improving the body's antioxidant capacity to protect the stomach.In mucosa,deer oil highly significantly reduced the levels of inflammatory factors TNF-αand IL-6 in the serum,could regulate the inflammatory factors produced by the damage of ethanol to the gastric mucosa,and reduced the mRNA expression of EPO and EPOR;rats in the butter pretreatment group,there were many slender bleeding bands on the surface of the gastric mucosa,the color was slightly darker,the cell arra
作者
夏蕴实
孙印石
刘畅
李志满
姜辉
王梓
XIA Yunshi;SUN Yinshi;LIU Chang;LI Zhiman;JIANG Hui;WANG Zi(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China;College of Food Science and Engineering,Changchun University,Changchun 130022,China;Jilin Teyan Pharmaceutical Co.,Ltd.,Jilin 132109,China)
出处
《食品工业科技》
CAS
北大核心
2021年第19期369-375,共7页
Science and Technology of Food Industry
基金
国家重点研发计划(2018YFC1706604
2018YFC1706605)
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)
中央级公益性科研院所基本科研业务费专项(1610342020018)。
关键词
鹿油
牛油
猪油
乙醇
胃粘膜损伤
deer oil
butter
lard
ethanol
gastric mucosal injury
