摘要
为得到高抗氧化活性的鲤鱼鳞胶原蛋白酶解液,试验以羟基自由基(·OH)和超氧阴离子自由基(O_(2)^(-)·)清除率作为主要检测指标,通过单因素试验研究酶解温度、pH值、加酶量、底物浓度、酶解时间对鱼鳞胶原蛋白酶解液抗氧化活性和水解度的影响。在此基础上,采用响应面分析法对酶解工艺条件进行优化。得到试验最优工艺为酶解时间4.4 h,酶解温度66.0℃,pH8.5,底物浓度20 mg/mL,加酶量4%。在此条件下,酶解液的·OH清除率为80.24%,O_(2)^(-)·清除率为65.34%,亚铁离子(Fe^(2+))螯合能力为59.73%。对比常用抗氧化剂,在O_(2)^(-)·清除能力方面,酶解液效果低于丁基羟基茴香醚(butylated hydroxyanisole,BHA)和抗坏血酸(P<0.05),但在·OH清除能力方面,酶解液已达到BHA和抗坏血酸的水平(P>0.05),在Fe2+螯合能力方面,酶解液显著优于BHA和抗坏血酸(P<0.05)。
Hydroxyl radical(·OH)and superoxide anion radical(O_(2)^(-)·)scavenging rates were used as the main indexes to obtain the collagenase hydrolysate of carp(Cyprinus carpio)scales with high antioxidant activity.The effects of enzymatic hydrolysis temperature,pH,enzyme dosage,substrate concentration,and hydrolysis time on the antioxidant activity and degree of hydrolysis of the hydrolysates were investigated through a single-factor experiment.The process conditions of enzymatic hydrolysis were optimized through response surface analysis.The results showed that the optimum conditions were as follows:the enzymolysis time was 4.4 h,enzymolysis temperature was 66.0℃,pH was 8.5,substrate concentration was 20 mg/mL,and enzyme amount was 4%.Under these conditions,the scavenging rates of the·OH,O_(2)^(-)·,and Fe^(2+)chelating ability were 80.24%,65.34%,and 59.73%,respectively.Compared to widely used antioxidants,the enzymolysis solution was lower than butylated hydroxyanisole(BHA)and ascorbic acid in terms of the O_(2)^(-)·scavenging ability(P<0.05)but reached the level of the BHA and ascorbic acid in the·OH scavenging ability(P>0.05).In terms of the Fe2+chelating ability,the enzymatic hydrolysates were significantly better than the BHA and ascorbic acid(P<0.05).
作者
于海洋
彭新颜
崔晓颖
常高坦
刘凯丽
YU Hai-yang;PENG Xin-yan;CUI Xiao-ying;CHANG Gao-tan;LIU Kai-li(Food Engineering,Shandong Business Institute,Yantai 264670,Shandong,China;College of Life Sciences,Yantai University,Yantai 264005,Shandong,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第18期40-48,共9页
Food Research and Development
基金
国家自然科学基金面上基金项目(32072141)
山东省重点研发计划项目(2019GSF108011)。
关键词
鲤鱼鳞
酶水解
响应面分析
工艺优化
抗氧化活性
carp scales
enzymatic hydrolysis
response surface methodology
process optimization
antioxidant activity
