摘要
【目的】探讨五味子木脂素6种单体成分对大鼠离体气管平滑肌张力的影响及其舒张气管平滑肌的作用机制。【方法】采用离体气管灌流法,观测终浓度为10^(-7)~10^(-3) mol/L的五味子醇甲、五味子醇乙、五味子甲素、五味子乙素、五味子丙素、五味子酯甲和体积分数1‰二甲基亚砜(DMSO,对照)对静息状态及乙酰胆碱(ACh)预收缩状态下大鼠离体气管环张力的影响。用L型Ca^(2+)通道阻滞剂维拉帕米(10^(-5) mol/L)、β-肾上腺素受体阻断剂普萘洛尔(10^(-5) mol/L)和体积分数1‰DMSO(对照)预孵育大鼠离体气管环15 min,然后依次加入浓度为10^(-5)~10^(-3) mol/L的五味子醇甲,观测维拉帕米和普萘洛尔对五味子醇甲舒张气管作用的影响。【结果】与对照比较,不同浓度的6种五味子木脂素单体对静息状态下的气管环张力均无显著影响(P>0.05)。五味子醇甲、五味子醇乙、五味子甲素和五味子乙素对ACh预收缩的气管环张力相较对照具有显著抑制作用,且呈剂量依赖性(P<0.001),最大抑制率分别为(92.12±3.28)%,(78.23±10.20)%,(62.95±12.93)%及(46.58±4.92)%;而五味子酯甲和五味子丙素对ACh预收缩气管环张力无明显影响(P>0.05)。与DMSO对照组相比,五味子醇甲对大鼠离体气管环的舒张作用可被维拉帕米明显减弱(P<0.001),最大抑制率降低了(26.24±8.42)%,但普萘洛尔对其作用无显著影响(P>0.05)。【结论】五味子醇甲、五味子醇乙、五味子甲素和五味子乙素可呈剂量依赖性地舒张大鼠离体气管平滑肌,其中五味子醇甲的作用机制可能与阻断L型Ca^(2+)通道有关。
【Objective】The aim of this study was to investigate effects of 6 monomers of lignans from Schisandra chinensis on the tension of isolated tracheal smooth muscle of rats and the relaxation mechanism.【Method】Trachea perfusion experiment in vitro was performed for understanding the effects of schisandrol A,schisandrol B,schisandrin A,schisandrin B,schisandrin C and schisantherin A with final concentration of 10^(-7)-10^(-3) mol/L and 1‰dimethy sulfoxide(DMSO,control)on tension of isolated tracheal ring under resting state and acetylcholine(ACh)pre-contraction state.The isolated rat tracheal rings were pre-incubated with L-type Ca^(2+) channel blocker verapamil(10^(-5) mol/L),β-adrenoceptor blocker propranolol(10^(-5) mol/L)and 1‰DMSO(control)for 15 min,then final concentration of 10^(-5)-10^(-3) mol/L schisandrol A was added to investigate the effects of verapamil and propranolol on tracheal relaxation of schisandrol A.【Result】Compared with DMSO control group,6 monomers of S.chinensis lignans at different concentrations had no significant effect on tension of tracheal ring at resting state(P>0.05).Schisandrol A,schisandrol B,schisandrin A and schisandrin B significantly inhibited the tension of tracheal rings pre-contracted with ACh in a dose-dependent manner(P<0.001)with maximum inhibition rates of(92.12±3.28)%,(78.23±10.20)%,(62.95±12.93)%and(46.58±4.92)%,respectively.Schisantherin A and schisandrin C had no significant effect(P>0.05).Compared with DMSO control group,the relaxation effect of schisandrol A on isolated rat tracheal ring was significantly reduced by verapamil(P<0.001)with maximum inhibition rate of(26.24±8.42)%,while propranolol had no significant effect(P>0.05).【Conclusion】Schizandrol A,schisandrol B,schisandrin A and schisandrin B can relax isolated tracheal smooth muscle of rats in a dose-dependent manner,and schisandrol A might relate to the blocking of L-type Ca^(2+) channel.
作者
陈丽华
许智莹
林程程
孙靖辉
李贺
陈建光
王春梅
CHEN Lihua;XU Zhiying;LIN Chengcheng;SUN Jinghui;LI He;CHEN Jianguang;WANG Chunmei(Editorial Department of Journal of Beihua University,Beihua University,Jilin,Jilin 132013,China;College of Pharmacy,Beihua University,Jilin,Jilin 132013,China)
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2021年第10期24-29,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
吉林省中医药科技项目(2020119)
北华大学研究生创新项目(2019017)。
关键词
五味子木脂素
五味子醇甲
气管平滑肌
气管舒张作用
lignans from Schisandra chinensis
schisandrol A
tracheal smooth muscle
tracheal relaxation
