摘要
从基因表达综合数据库下载GSE83514数据集,利用加权基因共表达网络分析(WGCNA)筛选与牛口蹄疫病毒感染的相关模块及枢纽基因,并对整个模块内的基因进行GO功能注释和KEGG通路分析。结果显示,通过构建WGCNA共表达网络,确定与牛口蹄疫病毒感染显著负相关的Darkred模块和显著正相关的Green模块为枢纽模块,它们的枢纽基因分别为MFSD4和RHOH。这两个模块基因共富集到20个生物学过程及14条KEGG信号通路,并且两个模块同时富集到DNA复制通路。本研究为探究牛口蹄疫病毒感染通路变化提供了生物信息学依据,筛选的枢纽基因MFSD4和RHOH有望成为抑制牛口蹄疫病毒感染的治疗靶标。
In this study,GSE83514 data set was downloaded from Gene Expression Omnibus(GEO)database,and Weighted Gene Co-Expression Network Analysis(WGCNA)was used to explore the related modules and hub genes of bovine foot-and-mouth disease virus(FMDV)infection.The results showed that through constructing WGCNA co-expression network,the genes of Darkred module and Green module were found to be negatively and positively related to bovine FMDV infection respectively.MFSD4 and RHOH were the hub gene of Darkred module and Green module,respectively.Twenty Gene Ontology(GO)terms and 14 Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways were enriched in those two modules.DNA replication signaling pathway was the only pathway enriched in both modules.The results of this study provided a bioinformatics basis for investigating the molecular mechanism of bovine FMDV infection,and the selected hub genes,MFSD4 and RHOH,were expected to be therapeutic biomarker for inhibiting the infection of FMDV.
作者
杨生海
刘西兰
张勇
YANG Shenghai;LIU Xilan;ZHANG Yong(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;China Agricultural Vet.Bio.Science and Technology Co.,Ltd.,Lanzhou 730046,China)
出处
《浙江农业学报》
CSCD
北大核心
2021年第9期1617-1624,共8页
Acta Agriculturae Zhejiangensis
基金
动物繁殖疾病防控协同创新团队(2018C-15)。
关键词
牛口蹄疫病毒
转录组测序
加权基因共表达
数据挖掘
枢纽基因
foot-and-mouth disease virus of bovine
RNA-seq
weighted gene co-expression network analysis
data excavation
hub gene
