摘要
目的:建立黄芪药材高效液相色谱指纹图谱分析方法。方法:采用高效液相色谱法,色谱柱:Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm),流动相A:乙腈-甲醇(90∶10),流动相B:0.2%甲酸溶液,梯度洗脱,流速:0.7 ml/min,柱温:30℃;进样量:10μl;检测波长:254 nm。结果:通过方法学考察及17批样品的测定,建立了黄芪药材的HPLC指纹图谱,确定了10个共有峰,3个产地17批黄芪药材特征峰的相似度在0.92以上,各产地黄芪药材有一定的差异性。主成分分析中前3个主成分累计贡献率为92.0%,说明这3个主成分包含了被分析样本的绝大部分信息。聚类分析按产地将17批黄芪药材聚为3类。结论:本文所建立的方法准确、可靠,可用于评价和控制不同产地黄芪药材的质量。
Objective:To establish the high-performance liquid chromatography(HPLC)fingerprint analysis method for Astragalus membranaceus.Methods:HPLC was performed on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5μm)with mobile phase A of acetonitrile-methanol(90∶10)and mobile phase B of 0.2%formic acid solution for gradient elution,at a flow rate of 0.7 ml/min,a column temperature of 30℃,a sample size of 10μl,and a detection wavelength of 254 nm.Results:The HPLC fingerprints of Astragalus membranaceus were established based on methodological analysis and measurement of 17 batches of samples,and a total of 10 common peaks were identified.The similarity of characteristic peaks was above 0.92 for the 17 batches of Astragalus membranaceus from three producing areas,and there was a certain difference between Astragalus membranaceus from different producing areas.The principal component analysis showed that the top three principal components had a cumulative contribution rate of 92.0%,suggesting that these three principal components contained most of the information of the samples analyzed.The cluster analysis showed that the 17 batches of Astragalus membranaceus were clustered into three categories based on producing area.Conclusion:The method established in this study is accurate and reliable and can be used for quality assessment and control of Astragalus membranaceus from different producing areas.
作者
张贵赋
廖鑫
龚年春
彭艳梅
ZHANG Guifu;LIAO Xin;GONG Nianchun;PENG Yanmei(Hunan Provincial Center for Drug Inspection,Changsha 410013,Hunan,China;Hunan Academy of Chinese Medicine,Changsha 410013,Hunan,China)
出处
《湖南中医杂志》
2021年第8期181-184,187,共5页
Hunan Journal of Traditional Chinese Medicine
基金
湖南省传统中药制剂质量评价和溯源工程技术研究中心项目(2018TP2035)。
关键词
黄芪
高效液相色谱法
指纹图谱
实验研究
Astragalus membranaceus
high-performance liquid chromatography
fingerprint
experimental study
