摘要
为建立不同采收期黄芪药材HPLC指纹图谱并测定5种成分含量,确定采收期对宁夏六盘山冷凉地区黄芪质量的影响,以乙腈甲醇混合液-醋酸缓冲盐梯度洗脱,检测波长260 nm,建立黄芪药材HPLC指纹图谱,以DAD检测器测定毛蕊异黄酮葡萄糖苷、芒柄花黄素、刺芒柄花苷、毛蕊异黄酮含量;以乙腈-水等度洗脱,ELSD检测器测定黄芪甲苷含量。结果显示,14批不同采收时间黄芪药材HPLC指纹图谱有15个共有峰,5月采收的黄芪毛蕊异黄酮葡萄糖苷与刺芒柄花苷含量较高,10月采收的黄芪毛蕊异黄酮葡萄糖苷与黄芪甲苷含量较高,5月与10月采收的黄芪指纹图谱共有峰总峰面积较大。在宁夏六盘山冷凉地区,黄芪宜在5月或10月采收,HPLC指纹图谱结合含量测定,能够客观、有效地评价不同采收时期黄芪质量。
To study the effect of harvesting time on the quality of Radix Astragali in the cold area of Liupan Mountain in Ningxia,we established the HPLC fingerprint and determined the content of 5 components of Radix Astragali.The HPLC fingerprint was established by gradient elution with acetonitrile-methanol and acetic acid buffer salt in 260 nm,and the contents of calycosin-7-glucoside,formononetin,ononin and calycosin were determined with DAD detector;the content of astragaloside Ⅳ was determined with acetonitrile and water isocratic elution and ELSD detector.The fingerprint of 14 batches of Radix Astragali from different harvesting time had 15 common peaks.The contents of calycosin-7-glucoside and ononin were higher in May,and the contents of calycosin-7-glucoside and astragaloside Ⅳ were higher in October,and the total peak area of the shared fingerprint peaks was relatively high in May and October.In the cold area of Liupan Mountain in Ningxia,Radix Astragali is preferably harvested in May or October,and HPLC fingerprint combined with content determination can objectively and effectively evaluate the quality of Radix Astragali at different harvesting periods.
作者
马燕
李芯国
毛福英
余建强
赵云生
赵建军
MA Yan;LI Xinguo;MAO Fuying;YU Jianqiang;ZHAO Yunsheng;ZHAO Jianjun(School of Pharmacy,Ningxia Medical University,Yinchuan 750004;School of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200;Ningxia Engineering and Technology Research Center for Modernization of Regional Characteristic Traditional Chinese Medicine,Yinchuan 750004;Key Laboratory of Hui Ethnic Medicine Modernization,Ministry of Education,Yinchuan 750004)
出处
《中国农学通报》
2022年第10期149-156,共8页
Chinese Agricultural Science Bulletin
基金
宁夏回族自治区重点研发计划(东西部科技合作)项目“宁夏六盘山地产特色药材深加工技术体系构建与示范”(2017BY079)
河北中医学院人才队伍建设项目(1020103022)。
关键词
黄芪
六盘山
指纹图谱
含量测定
采收期
Radix Astragali
Liupan Mountain
fingerprint
content determination
harvest time
