摘要
采用顶空—固相微萃取和气相色谱—质谱联用技术(HS–SPME–GC–MS)测定浙江楠(Phoebe chekiangensis)、闽楠(P.bournei)、紫楠(P.sheareri)、小叶桢楠(P.zhennan f.oblong)和大叶桢楠(P.zhennan f.elliptical)叶片挥发性成分,并比较浙江楠叶片在不同季节、不同方位和不同温度下的挥发性成分差异及其日变化规律。结果表明,5种楠属植物叶片共检测出80种化合物,其中浙江楠52种、闽楠55种、紫楠41种、小叶桢楠56种、大叶桢楠46种,(+)–β–瑟林烯、3–己烯–1–醇和β–榄香烯可能为浙江楠叶片香气的特征物质。在夏季和秋季浙江楠叶片中分别鉴定出70和64种化合物,其中夏季西方位叶片成分种类最多,而秋季北方位叶片成分种类最多。正交偏最小二乘法判别分析(OPLS-DA)表明南、北方位叶片成分差异大,而东、西方位叶片成分较相似。α–古巴烯和α–葎草烯等含量在不同温度培养下的浙江楠叶片中存在显著差异,其中15和25℃下挥发性成分相近,而45℃下的成分显著区别于其他温度。挥发性成分日变化分析发现浙江楠叶片α–古巴烯、β–荜澄茄烯和γ–摩勒烯等8种物质在12:00或14:00出现高峰,而β–石竹烯、右旋大根香叶烯和β–榄香烯等10种物质在12:00或14:00最低。研究结果可为浙江楠等楠属植物精油的开发利用及以楠属植物为主体林分的森林康养提供参考。
The Phoebe species are valuable ornamental trees,with abundant volatile compounds in the leaves.In order to determine the dynamic changes of volatile compounds in the leaves of five Phoebe species,the headspace-solid phase microextraction(SPME),separated and analyzed by gas chromatography-mass spectrometry(GC–MS).A total of 80 volatile compounds were identified from five Phoebe species,and 52,55,41,56,and 46 compounds were detected from P.chekiangensis,P.bournei,P.sheareri,P.zhennan f.oblong,and P.zhennan f.elliptical,respectively.A total of 70 and 64 volatile compounds were identified from four directions of P.chekiangensis leaves in summer and autumn,respectively.In summer,the maximum variety of compounds was detected in western leaves,while in the autumn the maximum variety was detected in northern leaves.Further,OPLS-DA analysis showed that the volatile compounds were differed between northern and southern leaves,while the western and eastern leaves shared similar compounds.Moreover,we identified six major components,includingα-copaene,andα-humulene significantly diverged in leaves grown under four temperatures.In particular,the compound contents under 45℃significantly differed from the other three temperatures.Additionally,the volatile components showed diurnal variation in P.chekiangensis leaves.Eight compounds includingα-copaene,β-cadinene andγ-muurolene peaked at 12:00 or 14:00,while ten compounds such asβ-caryophyllene,(+)-germacrene D andβ-elemene had the lowest content at 12:00 or 14:00.These results provided theoretical guidance on developing and utilizing essential oils from the leaves of P.chekiangensis and other Phoebe species,and the forest rehabilitation at the Phoebe principal stand.
作者
褚洒洒
范祥祯
邱肇凯
杨琪
张毓婷
王洋
徐晓珊
童再康
张俊红
CHU Sasa;FAN Xiangzhen;QIU Zhaokai;YANG Qi;ZHANG Yuting;WANG Yang;XU Xiaoshan;TONG Zaikang;ZHANG Junhong(Zhejiang A&F University,State Key Laboratory of Subtropical Silviculture,Hangzhou 311300,China;Forestry Working Station of Hushan in Suichang County,Lishui,Zhejiang 323308,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2021年第8期1552-1564,共13页
Acta Horticulturae Sinica
基金
浙江省农业新品种选育重大专项(2021C2070-10)
浙江省重点研发计划项目(2021C02054)。
