摘要
探讨梨叶片解剖结构与抗寒性的关系,筛选抗寒性状指标,为梨抗寒品种选育过程中亲本的选择、杂交后代的早期鉴定以及品种引种栽培区划提供依据。利用石蜡切片技术测定13个梨品种(系)的叶片组织结构指标,基于变异系数、采用相关性分析和聚类分析法筛选叶片解剖结构中具有代表性的典型指标,应用隶属函数法结合田间自然冻害调查情况,综合评价各品种(系)的抗寒性。结果显示,13个梨品种(系)的叶片横切面均由上表皮、栅栏组织、海绵组织和下表皮构成,为典型的异面叶。栅栏组织细胞层数为2~3层。不同梨品种(系)叶片解剖结构总体上相似,但在量化上有一定的差异。结合聚类分析和相关指数,筛选出组织结构紧密度、海绵组织厚度和叶片厚度3项叶片抗寒性解剖结构的典型指标,13个梨品种(系)的抗寒性表现为苹果梨>新梨6号>鸭梨>新梨9号>84-1-1>新梨7号>早酥梨>新梨8号>砀山酥梨>新梨11号>新梨10号>新梨1号>库尔勒香梨。综上,组织结构紧密度、海绵组织厚度和叶片厚度是影响梨品种(系)抗寒性能的重要指标,根据叶片的组织结构解剖特性,利用隶属函数法结合田间冻害表现可以较好地反映不同梨品种(系)的抗寒能力。
In order to provide some references for the selection of parents,early identification of hybrid progeny during the breeding of cold‑resistant pear varieties and the regionalization of pear introduction and cultivation,relationship between leaf anatomic structure and cold tolerance of pear was discussed and the cold tolerance evaluation indexes were screened.In this study,8 anatomical structure indexes were measured of 13 pear varieties(strains)by using paraffin section methods.Typical representative indexes of leaf anatomical structure were screened via coefficient of variation,correlation analysis and hierarchical cluster analysis,and comprehensive evaluation on cold resistance of each varieties(strains)was conducted by the subordinate function method combined with the investigation of natural freezing injury in the field.The results showed that pear leaf transection of 13 varieties(strains)was composed of upper and lower epidermis,palisade tissue and spongy tissue and the leaves belonged to typical bifacial leaf.Cell layer number of palisade tissue was most 2—3 layers.The leaf anatomic structure of different pear varieties(strains)was similar on the whole,but there were some quantitative differences.Combined with cluster analysis and correlation index,three typical indexes of the cold‑resistant anatomical structure of leaves were selected,which were tightness of palisade tissue,sponge tissue thickness and leaf thickness.The cold resistance of the 13 varieties(strains)showed Pingguoli>Xinli No.6>Yali>Xinli No.9>84‑1‑1>Xinli No.7>Zaosuli>Xinli No.8>Dangshansuli>Xinli No.11>Xinli No.10>Korla fragrant pear.Main indexes of leaf anatomical structure that affect the cold resistance of pear varieties(strains)are tightness of palisade tissue,sponge tissue thickness and leaf thickness.According to the anatomical characteristics of leaf tissue structure,the method of subordinate function combined with the performance of field freezing injury can better reflect the cold resistance of different pear varieties(stra
作者
位杰
张琦
林彩霞
蒋媛
WEI Jie;ZHANG Qi;LIN Caixia;JIANG Yuan(Agricultural Scientific Institute of 2nd Division of Xinjiang Production and Construction Corps,Tiemenguan 841005,China;College of Plant Science,Tarim University,Alar 843300,China)
出处
《河南农业科学》
北大核心
2021年第8期103-112,共10页
Journal of Henan Agricultural Sciences
基金
新疆生产建设兵团中青年科技创新领军人才计划项目(2019CB032)
新疆生产建设兵团重点领域科技攻关项目(2018AB035)
河北省科技厅科技计划项目(16246327D)
新疆生产建设兵团第二师科技攻关与成果转化项目(2017NYGG01)。
关键词
梨
叶片
解剖结构
抗寒性
综合评价
Pear
Leaf
Anatomical structure
Cold resistance
Comprehensive evaluation
