摘要
【目的】研究不同低温胁迫处理下,2个石榴品种枝条生理指标的动态响应差异,旨在揭示不同石榴品种的抗寒特性。【方法】以新疆主栽区的叶城酸石榴(耐寒性强)和引种的突尼斯软籽石榴1年生枝条为试验材料,采用人工模拟低温环境的方法,观测经低温胁迫后各石榴品种在不同低温条件(-4、-6、-8、-10、-12℃)不同持续时间(6、12、18、24、30、36、42、48 h)下枝条相对电导率、超氧化物歧化酶(superoxide dismutase,SOD)活性、过氧化氢酶(catalase,CAT)活性、过氧化物酶(peroxidase,POD)活性、可溶性蛋白含量、可溶性糖含量等生理指标的变化情况,以确定不同石榴品种在各种低温条件下的最长耐受时间。【结果】随着胁迫温度的下降,突尼斯软籽和叶城酸1年生枝条的相对电导率、SOD活性、CAT活性、POD活性、可溶性蛋白含量、可溶性糖含量均呈上升趋势;同一低温下SOD活性、CAT活性、POD活性、可溶性蛋白含量随着低温持续时间延长呈现先升高后下降趋势,相对电导率和可溶性糖含量均呈上升趋势。随着胁迫温度的降低,突尼斯软籽和叶城酸对低温的忍耐时间缩短。突尼斯软籽幼苗在-4℃下能忍受48 h以上的低温胁迫,-6℃下能忍受36 h,-8℃下能忍受30 h,-10℃下能忍受18 h,-12℃下能忍受12 h,而叶城酸幼苗在-4℃和-6℃下能忍受48 h以上的低温胁迫,-8℃下能忍受42 h的低温胁迫,-10℃下忍受36 h,-12℃下忍受30 h。【结论】叶城酸的抗寒性显著强于突尼斯软籽,抗寒机制主要与枝条的抗氧化和渗透调节能力有关。研究结果为其他地区的石榴引种、栽培和推广应用提供参考和依据。
【Objective】The study investigated the dynamic variations in physiological indices in two varieties of pomegranate subjected to various low temperature stress treatments,with the aim to elucidate the cold resistance of Tunisia Soft-seed pomegranate.【Methods】Using annual branches of Yechengsuan pomegranate(cold-resistant)from the main planting area in Xinjiang and the introduced Tunisia Softseed pomegranate as experimental materials,artificially simulated low-temperature conditions were employed.The membrane leakage of branches,activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),soluble proteins,and soluble sugars were analyzed after low-temperature stress under different cold temperatures(-4℃,-6℃,-8℃,-10℃,and-12℃)and durations(6 h,12 h,18 h,24 h,30 h,36 h,42 h,and 48 h).The annual branches kept at room temperature served as the control.Physiological and biochemical analyses were performed on the samples 8 hours after cold temperature exposure ended.The determination of membrane leakage was the carried out immediately.For the determination of the other indicators,the branches were flash-frozen in liquid nitrogen after labelling and then stored in a-80℃freezer for upcoming measurements.The tough bark part of the branch was sampled and three replicated were set for each measurement.The membrane leakage was measured using the electricity conductivity method.The activity of superoxide dismutates(SOD)was measured using the photochemical reduction method with nitrogen blue tetrazole(NBT).The peroxidase(POD)activity was measured using the guaiacol method.The activity of catalase(CAT)was measured by UV absorption method.The soluble protein content was determined using the Coomassie Brilliant Blue method.The soluble sugar content was determined by the anthrone colorimetric method.Correlation analysis among various physiological indicators was conducted.【Results】The result showed that as the temperature decreased the change patterns of physiological and biochemical indicators of Tuni
作者
黄梦真
柴亚倩
关思慧
杨元玲
宋娟
唐诚
杨磊
刁明
HUANG Mengzhen;CHAI Yaqian;GUAN Sihui;YANG Yuanling;SONG Juan;TANG Cheng;YANG Lei;DIAO Ming(College of Agriculture,Shihezi University,Shihezi 832003,Xinjiang,China;College of Forestry and Landscape Architecture,Xinjiang Agricultural University,Urumqi 830000,Xinjiang,China;Institute of Horticultural Crops,Xinjiang Academy of Agriculture Sciences,Urumqi 830000,Xinjiang,China)
出处
《果树学报》
CAS
CSCD
北大核心
2024年第6期1150-1159,共10页
Journal of Fruit Science
基金
新疆生产建设兵团重大科技项目(2021AB015)。
关键词
石榴
抗寒性
低温胁迫
抗氧化酶
Pomegranate
Cold resistance
Low temperature stress
Antioxidant enzymes
