摘要
目的探讨褪黑素对爆震伤诱导小鼠肺损伤的保护作用及机制。方法将30只C57BL/6小鼠随机分为假手术(Sham)组、爆震伤(Blast)组及爆震伤+褪黑素(Blast+Mel)组。爆震伤后48 h收集小鼠肺组织标本。采用苏木精-伊红(HE)染色、酶联免疫吸附实验(ELISA)和蛋白质印迹实验(Western blotting)检测褪黑素对肺爆震伤的保护作用。结果与Blast组小鼠相比,褪黑素能够显著降低小鼠肺湿重/干重比和血清白细胞介素-4(IL-4)及肿瘤坏死因子-α(TNF-α)的表达水平;改善小鼠肺组织病理学变化;降低肺组织白细胞介素-6(IL-6)、白细胞介素-17(IL-17)和白细胞介素-23(IL-23)表达水平。同时,褪黑素明显抑制爆震伤导致的小鼠重组人Semaphorin 7a蛋白(Sema7a)和人胎球蛋白A(Ahsg)蛋白表达;显著降低小鼠肺组织P38蛋白(p38)和核因子κB(NF-κB)通路蛋白表达。结论褪黑素可能通过抑制p38/NF-κB信号通路发挥抗炎活性,进而对爆震伤诱导的肺损伤进行保护。
Objective To investigate the protective effect and mechanism of melatonin against blast-induced lung injury.Methods Thirty C57 BL/6 mice were randomly divided into three groups:sham group,blast group,and blast+melatonin group.The lung tissue samples of mice were collected 48 hours after blast injury.The protective effect of melatonin on lung blast injury was detected by hematoxylin and eosin(HE)staining,enzyme-linked immunosorbent assay,and western blotting.Results Compared with the blast injury group,melatonin significantly reduced the lung wet weight/dry weight ratio and serum IL-4 and TNF-αexpression,improved the pathological changes of lung tissue,and decreased the expression of interleukin(IL)-6,IL-17,IL-23 in lung tissues of the blast+melatonin group.Meanwhile,melatonin significantly inhibited Sema7 a and Ahsg protein expression and reduced the levels of p38 and NF-κB protein.Conclusion Melatonin may exert anti-inflammatory effects by inhibiting the p38/NF-κB signaling pathway,protecting against lung injury induced by blast injury.
作者
施琳
赵剑
SHI Lin;ZHAO Jian(Department of Pharmacology,Life Science and Biopharmaceutical Institution,Shenyang Pharmaceutical University,Shenyang 110016,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2021年第9期804-808,共5页
Journal of China Medical University
基金
辽宁省自然科学基金(20170540947)。
关键词
爆震伤
褪黑素
肺损伤
炎症
blast injury
melatonin
lung injury
inflammation
