摘要
土霉素(OTC)作为一种广谱类抗生素,被广泛用于畜禽养殖和水产养殖业.大量排放到水体和土壤中的OTC会在环境中不断积累,危害生态环境和人类健康.利用分子印迹技术,以邻苯二胺为功能单体,使用电聚合法在氧化石墨烯-聚乙烯亚胺复合材料(RGO-PEI)修饰的玻碳电极表面合成OTC分子印迹聚合物薄膜,制备OTC分子印迹电化学传感器.在5.0 mmol/L K_(3)[Fe(CN)_(6)]/0.1 mol/L KCl溶液中,采用循环伏安法和线性扫描伏安法,考察OTC分子印迹电化学传感器的响应性能.在最佳检测条件下,传感器的峰电流差值与OTC浓度在1.0~100.0μmol/L的范围内呈现良好的线性关系,检出限为0.33μmol/L(S/N=3).该传感器表现出良好的重现性和稳定性,对干扰物氯霉素、金霉素和盐酸多西环素几乎无响应.在实际水样中所测得的OTC加标回收率为100.4%~102.6%.该研究为OTC的检测提供了一种简单、经济、高效的电化学方法.
Oxytetracycline(OTC),as a broad-spectrum antibiotic,is widely used in livestock and poultry breeding and aquaculture.A large amount of OTC discharged into water and soil will accumulate continuously in the environment and endanger the health of human and environment.OTC molecularly imprinted electrochemical sensor was constructed by the electropolymerization of a molecularly imprinted poly-o-phenylenediamine film on a reduced graphene oxide-polyethyleneimine complex(RGO-PEI)modified glassy-carbon electrode.The response performance of OTC sensor was investigated by cyclic voltammetry and linear sweep voltammetry in 5.0 mmol/L K_(3)[Fe(CN)_(6)]/0.1 mol/L KCl solution.Under the optimal detection conditions,the peak current difference between the sensor and OTC concentration is 1.0~100.0μmol/L with a detection limit of 0.33μmol/L(S/N=3).The sensor exhibited good repeatability and stability,and almost no response to interfering substances such as chloramphenicol,chlortetracycline and doxycycline hydrochloride.The recoveries of OTC were 100.4%~102.6%.This study provides a simple,economical and efficient electrochemical method for the detection of OTC.
作者
王慧
高原
韩双
WANG Hui;GAO Yuan;HAN Shuang(Shenyang University of Chemical Technology,Shenyang 110142,China)
出处
《沈阳化工大学学报》
CAS
2021年第2期107-113,共7页
Journal of Shenyang University of Chemical Technology
基金
辽宁省教育厅一般项目(L_(2)016022)。
关键词
土霉素
邻苯二胺
分子印迹
聚乙烯亚胺
电化学传感器
oxytetracycline
o-phenylenediamine
molecularly imprinting
polyethyleneimine
electrochemical sensor
