摘要
为研究红鳍东方鲀(Takifugu rubripes)不同生长阶段生长发育差异的分子机制,基于13与15月龄红鳍东方鲀的脑与肌肉组织转录组数据,以及在两时期间KEGG通路分析中差异显著的MAPK信号通路,结合基因互作分析对这期间发生的生物过程进行生物信息学方面的关联预测。结果表明:MAPK信号通路通过调节fos和jun及其下游基因,促进这期间肌肉和骨骼发育,使红鳍东方鲀在两时期间生长。而fos和jun在两组织均上调,但在脑中抑制fos和jun的基因上调程度更高,而在肌肉中则较低。MAPK信号通路介导的egr 1、c/ebpβ、ctgf等基因与骨骼发育相关,在此期间可能伴随着红鳍东方鲀骨骼的生长。MAPK信号通路还调节tgf-β、ctgf、pai 1等与组织纤维化相关的基因,在转录层面上纤维化过程在肌肉中持续而在脑中减弱,可能促进了肌肉与骨骼的发育。MAPK信号通路还可能影响脂质代谢,即脑中γ-氨基丁酸相关基因下调有促进脂肪积累的作用,该过程伴随着基础代谢降低。肌肉中核受体亚家族D组(nr 1 d)基因的下调促进了脂质吸收,mstn b基因可能调节了这个过程。
In order to investigate the molecular mechanisms underlying the differences in growth between the different growth stages of Takifugu rubripes,this study combined gene interaction analysis with transcriptomic data from brain and muscle tissues of 13-and 15-month-old T.rubripes and MAPK signaling pathways that differed significantly in the KEGG pathway analysis between the two stages to predict the biological processes that occurred during these periods.And bioinformatic correlation prediction was performed.The results showed that MAPK signaling pathway promoted muscle and bone development during these periods by regulating fos and jun and their downstream genes,which led to the growth of T.rubripes during the two-stage period.While fos and jun were upregulated in both tissues,the genes that repress fos and jun were more upregulated in the brain and less in the muscle.MAPK signaling pathway-mediated genes such as egr 1,c/ebpβ,and ctgf were found to be associated with skeletal development,and may accompany skeletal growth of T.rubripes during these periods.MAPK signaling pathway also regulates tgf-β,ctgf,pai 1 and other genes associated with tissue fibrosis,and at the transcriptional level the fibrosis process persists in muscle and is diminished in brain,possibly promote the development of muscle and bone,and sort out its possible intergenic interaction process.MAPK signaling pathway may also affect lipid metabolism,while downregulation ofγ-aminobutyric acid-related genes in brain has a role in promoting lipid accumulation,a process that may be accompanied by a decrease in basal metabolism,while downregulation of nuclear receptor subfamily D(nr 1 d)genes in muscle promotes lipid uptake,and mstn b genes may regulated this process.
作者
王冠
胡子文
周金旭
王盛南
李鑫
仇雪梅
王秀利
WANG Guan;HU Zi wen;ZHOU Jin xu;WANG Sheng nan;LI Xin;QIU Xue mei;WANG Xiu li(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)
出处
《河北渔业》
2021年第9期1-8,13,F0002,F0003,共11页
基金
国家重点研发计划课题(2019YFD0900503)
财政部和农业农村部:国家现代农业产业技术体系(CARS-47)
大连市科技创新基金项目(2018J12SN070)。
