摘要
红鳍东方鲀(Takifugu rubripes)是中国北方重要的海水养殖鱼类。本研究通过Illumina测序技术对13月龄和15月龄红鳍东方鲀的脑组织和肌肉组织共12个样本进行了转录组测序与分析。结果表明:在脑组织样本中平均共得到5510万条原始reads,在肌肉组织样本中平均共得到5482万条原始reads。在15月龄相对13月龄脑(BB15 vs BB13)中筛选到554个差异基因,其中234个上调表达基因,320个下调表达基因。15月龄相对13月龄肌肉(MB15 vs MB13)中筛选到380差异基因,其中253个上调表达基因,127个下调表达基因。GO富集结果将差异基因分为生物过程、细胞组成、分子功能三个层面。根据KEGG代谢通路分析,在BB15 vs BB13中,注释到163个差异基因,共富集到82条通路上,其中MAPK信号通路和神经信号传递通路的富集程度最高。在MB15 vs MB13中注释到138个差异基因,富集到97条通路上,其中也是MAPK信号通路富集程度最高。共筛选出3个可能与生长相关基因MSTN2、IER2、C-FOS。研究结果为今后开展红鳍东方鲀的基因研究提供了有价值的参考信息,也为改善养殖红鳍东方鲀的生长、抗逆和抗病能力的研究提供科学依据。
Takifugu rubripes is an important marine aquaculture fish in northern China.In this study,12 samples of brain and muscle tissue from 13-month-old and 15-month-old Takifugu rubripes were sequenced and analyzed by Illumina.The results showed that a total of 55.1 million original reads were obtained in brain,and an average of54.82 million original reads were obtained in muscle.Comparison analysis resulted in 554 differential expressed genes between 15-month-old and 13-month-old brains(BB15 vs BB13),of which 234 were up-regulated and 320 were down-regulated.And 380 differential expressed genes were screened between 15-month-old and 13-monthold muscle(MB15 vs MB13),of which 253 were up-regulated and 127 were down-regulated.The results of the Go divided the differential genes into biological processes,cell components,and molecular functions.According to KEGG metabolic pathway analysis,163 differential genes were annotated and enriched in 82 pathways from BB15 vs BB13.The Mitogen-activated protein kinase pathway and the MAPK pathway were the most enriched.And 138 differential genes were annotated and enriched on 97 pathways from MB15 vs MB13.The MAPK pathway enriched levels were the highest.A total of 3 growth-related genes MSTN2,IER2 and c-fos were screened.The research results provided valuable reference information for the future genetic research of Takifugu rubripes,and laid a foundation for the research on improving the growth,stress resistance and disease resistance of farmed Takifugu rubripes.
作者
王盛南
胡子文
余云登
马文超
杨晓
仇雪梅
王秀利
Wang Shengnan;Hu Ziwen;Yu Yundeng;Ma Wenchao;Yang Xiao;Qiu Xuemei;Wang Xiuli(College of Fisheries and Life Science,Dalian Ocean University,Dalian,116023)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第2期607-614,共8页
Genomics and Applied Biology
基金
大连市科技创新基金项目(2018J12SN070)
国家海水鱼产业技术体系岗位科学家项目(CARS-47)共同资助。
关键词
红鳍东方鲀
脑组织
肌肉组织
高通量测序
转录组
Takifugu rubripes
Brain tissues
Muscle tissues
High-throughput sequencing
Transcriptome
