摘要
目的探究微小RNA(miR)-145-5p与脓毒症急性肺损伤(ALI)的相关性。方法将60只雄性BALB/c小鼠随机分为对照组30只和ALI组30只,通过呼吸道滴注脂多糖(LPS)的方式建立小鼠脓毒症ALI模型。检测两组小鼠肺组织中miR-145-5p和AMP活化蛋白激酶(AMPK)蛋白水平并进行比较。采用Pearson相关分析评估ALI小鼠肺组织miR-145-5p与AMPK蛋白、支气管肺泡灌洗液(BALF)中炎性因子水平的相关性。将人正常肺上皮细胞BEAS-2B分为NC组、mimic组和mimic+AMPK组,比较3组细胞中miR-145-5p和AMPK水平。结果ALI组小鼠肺组织miR-145-5p水平、BALF中TNF-α和IL-6水平均高于对照组,而肺组织中AMPK蛋白水平低于对照组(P<0.05)。脓毒症ALI小鼠肺组织miR-145-5p与AMPK蛋白水平呈负相关,与BALF中TNF-α、IL-6水平均呈正相关(P<0.05)。mimic组、mimic+AMPK组细胞中miR-145-5p水平均高于NC组(P<0.05)。mimic组细胞中AMPK mRNA和蛋白水平均低于NC组,mimic+AMPK组细胞中AMPK mRNA和蛋白水平均高于mimic组(P<0.05)。结论在脓毒症ALI小鼠模型中,miR-145-5p的升高与炎性反应有关,且miR-145-5p可能通过靶向抑制AMPK的水平调节炎性反应,参与脓毒症引起的炎性反应。
Objective To explore the correlation between microRNA(miR)-145-5p and acute lung injury(ALI)in sepsis.Methods Sixty mice were randomly divided into control group(n=30)and ALI group(n=30).ALI model of mouse sepsis was established by instilling lipopolysaccharide(LPS)through the respiratory tract.MiR-145-5p and AMP-activated protein kinase(AMPK)protein levels in lung tissue were detected and compared.Pearson correlation analysis was used to evaluated the correlation between miR-145-5p and AMPK protein in lung tissue,inflammatory factors levels in bronchoalveolar lavage fluid(BALF).Human normal lung epithelial cells BEAS-2B were divided into NC group,mimic group and mimic+AMPK group,and the levels of miR-145-5p and AMPK in the 3 groups were compared.Human normal lung epithelial cells BEAS-2B were divided into NC group,mimic group and mimic+AMPK group,and miR-145-5p and AMPK levels in 3 groups were compared.Results MiR-145-5p level in lung tissue and TNF-α,IL-6 levels in BALF of mice in ALI group were higher than those in control group,while AMPK protein level in lung tissue was lower than those in control group(P<0.05).MiR-145-5p in lung tissue of sepsis ALI mice was negatively correlated with AMPK protein level,and positively correlated with TNF-α and IL-6 levels in BALF(P<0.05).MiR-145-5p levels in cells of mimic group and mimic+AMPK group were higher than those of NC group(P<0.05).AMPK mRNA and protein levels in cells of mimic group were lower than those of NC group,AMPK mRNA and protein levels in cells of mimic+AMPK group were higher than those of mimic group(P<0.05).Conclusion In sepsis ALI mouse model,the increase of miR-145-5p is related to inflammatory response,and miR-145-5p may regulate inflammatory response through targeted inhibition of AMPK level and participate in inflammatory response caused by sepsis.
作者
邓慧兰
韩文慧
王辉
王洁
Deng Huilan;Han Wenhui;Wang Hui;Wang Jie(Department of Respiratory Ⅰ,Qingdao Jiaozhou Central Hospital,Qingdao 266300,China;不详)
出处
《临床内科杂志》
CAS
2021年第8期552-555,共4页
Journal of Clinical Internal Medicine
