摘要
为了明确盐胁迫下甜菜是否存在自噬现象及其与植株耐盐生理特性的关系,以耐盐型甜菜品种LS2004和盐敏感型甜菜品种KWS7125为试验材料,研究在不同盐梯度处理下甜菜的自噬发生情况、活性氧含量、渗透调节物质含量及其之间的作用关系。结果表明:盐胁迫可诱导甜菜幼苗体内自噬现象的发生,自噬参与了甜菜幼苗对盐胁迫的防御过程,具体表现为随着盐浓度升高自噬作用增强,在300 mmol/L盐胁迫条件下,LS2004的自噬作用最强、自噬体数最多,为KWS7125的1.22倍。KWS7125的活性氧含量高于LS2004,在200 mmol/L处理下二者的过氧化氢含量达到峰值,分别较对照增加了40.06%及41.54%。在各处理下,KWS7125的超氧阴离子含量与相对电导率均高于LS2004。LS2004的可溶性蛋白含量、脯氨酸含量及可溶性总糖含量均随着盐浓度的升高而呈现先上升后下降的变化规律,但与KWS7125之间变化规律存在差异。综上,盐胁迫下甜菜幼苗存在自噬现象,并且甜菜幼苗可通过自噬作用、缓解氧化损伤与渗透调节物质含量等综合作用来提高甜菜耐盐能力。
In order to clarify whether existed autophagy phenomenon in sugar beet under salt stress and its relationship with plant salt-resistant physiological characteristics,the self-phage occurrence,reactive oxygen species content,osmosis regulating substance content and their relationship under different salt gradients were studied with salt-resistant sugar beet variety LS2004 and salt-sensitive sugar beet variety KWS7125 as experimental materials.The results showed that salt stress could induce autophagy in sugar beet seedlings.Autophagy was involved in the defense process of sugar beet seedlings against salt stress.The specific manifestation was that autophagy increased with the increase of salt concentration.Under the salt stress condition of 300 mmol/L,LS2004 had the strongest autophagy and the largest number of autophagosomes,which was 1.22 times that of KWS7125.The hydrogen peroxide content of KWS7125 and LS2004 reached the peak under the treatment of 200 mmol/L,which were increased by 40.06%and 41.54%respectively compared with that of the control.Under each treatment,the superoxide anion content and relative electrical conductivity of KWS7125 were higher than those of LS2004.The soluble protein content,proline content and total soluble sugar content of LS2004 firstly increased and then decreased with the increase of salt concentration,but there were differences in change rules with KWS7125.In summary,autophagy existed in sugar beet seedlings under salt stress,and the comprehensive effect of autophagy,alleviating oxidative damage,and osmotic adjustment substance content could be used by sugar beet seedlings to improve the salt tolerance of sugar beet.
作者
万雪
荆文旭
魏磊
邢旭明
史树德
WAN Xue;JING Wenxu;WEI Lei;XING Xuming;SHI Shude(Agricultural College,Inner Mongolia Agricultural University,Hohhot 010019,China)
出处
《华北农学报》
CSCD
北大核心
2021年第4期90-95,共6页
Acta Agriculturae Boreali-Sinica
基金
国家糖料产业技术体系赤峰综合试验站(CARS-170702)。
关键词
甜菜
耐盐性
自噬
活性氧
生理特征
Sugar beet
Salt tolerance
Autophagy
Active oxygen
Physiological characteristics
