摘要
目的通过生物信息学分析耐力运动对健康人外周血微RNA(miRNA)表达谱的影响,预测靶基因及信号通路。方法从公共基因芯片数据平台(GEO)下载miRNA基因芯片数据集GSE133910,此芯片数据为对23例经8周耐力运动后的健康受试者外周血miRNA进行检测分析。筛选耐力运动后的差异表达miRNA,对差异表达的miRNA靶基因进行预测及通路富集分析,构建蛋白质相互作用网络。结果8周耐力运动后健康受试者外周血miRNA中共有2个显著上调,6个显著下调。经数据库预测出251个可能的靶基因,GO分析显示主要参与离子通道结合、突触后致密蛋白结构域结合和mRNA结合等分子功能;KEGG分析显示靶基因主要涉及肿瘤信号通路、能量代谢通路、Wnt信号通路和甘油磷脂代谢通路。关键基因包括PRKACA、SMURF1、RHOA、GNAO1、CBL、UBE2D2、GSK3B、GNAQ、BCL2L1和RRAS2。结论耐力运动可以显著改变外周血miRNA表达谱,差异表达的miRNA可能通过多通路发挥作用。
Objective To analyze the effect of endurance exercise on the microRNA(miRNA)expression profile in peripheral blood from healthy individuals using bioinformatics,and to predict target genes and regulated signaling pathways.Methods The miRNA gene chip data set GSE133910 was downloaded from the public gene chip data platform(GEO).This chip data was used to identify miRNAs in peripheral blood from 23 healthy subjects,and analyze the effects of 8 weeks of endurance exercise on their expression.Differentially expressed miRNAs due to endurance exercise were screened for,miRNA target genes were predicted and pathway enrichment analysis for these target genes was performed.Protein interaction networks were also constructed.Results After 8 weeks of endurance exercise,two miRNAs were significantly up-regulated,and 6 were significantly down-regulated in peripheral blood of healthy subjects.The database predicted 251 possible target genes.GO analysis showed that they are mainly involved in ion channel binding,post-synaptic compact pro-tein domain binding,and mRNA binding.KEGG analysis found that target genes are mainly involved in tumor signaling pathways,energy metabolism pathways,the Wnt signaling pathway,and the glycerophospholipid metabolism pathway.Key genes included PRKACA,SM URF1,RHOA,GNAO1,CBL,UBE2D2,GSK3B,GNAQ,BCL2L1,and RRAS2.Conclusion This study found that endurance exercise can significantly change the expression profile of peripheral blood miRNAs,and the differentially expressed miRNAs may affect physiolo-gy through multiple pathways.
作者
孙庭汉
刘美
孙晓
SUN Tinghan;LIU Mei;SUN Xiao(Section of Sports,China Medical University,Shenyang 110122,China;Department of Ion Channel Pharmacology,School of Pharmacy,China Medical University,Shenyang 110122,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2021年第8期737-741,751,共6页
Journal of China Medical University
