摘要
几丁质是自然界含量丰富的多糖,难溶于水,常被作为废弃物丢弃,造成资源浪费和环境污染。然而,其水解产物N-乙酰氨基葡萄糖(GlcNAc)是一种重要的功能氨糖类化合物,广泛应用于医药、保健及护肤品等领域,市场需求量大。因此,将几丁质转换为高附加值的GlcNAc具有重要意义。几丁质酶可专一性水解几丁质产生GlcNAc,用于GlcNAc的酶法制备,从而替代化学加工方法,降低环境污染,提高产品质量。本文介绍了微生物来源几丁质酶的特点与分类,重点阐述了微生物来源的几丁质内切酶、几丁二糖外切酶及β-N-乙酰氨基葡萄糖苷酶在几丁质降解生产GlcNAc过程中的作用、方式和产率,这将为酶法生产GlcNAc提供一定的借鉴。
Chitin is the second most abundant carbohydrate polymer in nature,but often discarded as wastes.N-acetylglucosamine(GlcNAc),the final hydrolysate of chitin,is an important functional amino sugar compound that can be used in medicine,healthcare,and skin care products with a great demand.Therefore,it is of great significance to convert chitin to GlcNAc with high value added.Chitinase can specifically hydrolyze chitin to produce high value-added N-acetylglucosamine,to replace chemical processing strategy,thus reducing environmental pollution and improving product quality.This review briefly introduces specific features and classification of microbial chitinases.Then,the roles,mode and yield of endochitinase,chitobiosidase,and β-N-acetylglucosidase in the production of GlcNAc from chitin in recent years are elaborated,to provide references for enzymatic production of GlcNAc.
作者
周玉玲
蒋思婧
贺妮莎
张桂敏
Yuling Zhou;Sijing Jiang;Nisha He;Guimin Zhang(State Key Laboratory of Biocatalysis and Enzyme Engineering,Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources,School of Life Sciences,Hubei University,Wuhan 430062,Hubei Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2021年第8期2192-2204,共13页
Acta Microbiologica Sinica
基金
国家重点研发计划(2018YFA0901100)
国家自然科学基金(31970059)
2016武汉黄鹤英才人才项目。
