摘要
为了了解甘孜州藏猪源猪流行性腹泻病毒(Porcine epidemic diarrhea virus, PEDV)S基因的分子特征,试验对2018年1—12月份采自甘孜州4个县12个藏猪养殖场的144份腹泻粪便样本进行PEDV的分子检测和S基因的分子特征研究。结果表明:144份腹泻粪便样本中PEDV核酸阳性率为43.06%(62/144),12个藏猪场中PEDV猪场阳性率为91.67%(11/12);经PCR扩增及克隆共获得7株藏猪源PEDV毒株完整S基因,长度为4 146~4 158 bp;7株藏猪源PEDV毒株S基因彼此之间的核苷酸序列同源性为95.7%~99.9%,推导氨基酸序列相似性为93.8%~99.9%,与参考毒株S基因核苷酸序列同源性为93.1%~98.3%,氨基酸序列相似性为91.0%~98.1%;7株藏猪源PEDV毒株S基因在G1和G2两个大支之间形成了两个独立的分支,可能代表两种新的S基因型,在S蛋白的COE中和抗原表位上首次发现了L535I氨基酸突变。说明四川省甘孜州藏猪源PEDV感染率高,且S基因表现出独特的遗传进化特征。
In order to understand the molecular characteristics of S gene of Tibetan Porcine epidemic diarrhea virus(PEDV) in Ganzi Prefecture, in this study, 144 fecal samples from 12 Tibetan Pig farms in four counties of Ganzi Prefecture were collected from January 2018 to December 2018 for PEDV detection and molecular characterization of S gene. The results showed that the positive rate of PEDV was 43.06%(62/144), the positive rate of PEDV in 12 Tibetan Pig farms was 91.67%(11/12), and complete S gene sequences of seven PEDV strains from Tibetan pigs were obtained by PCR amplification and cloning. The length of S genes ranged from 4 146 to 4 158 bp. The nucleotide similarity of seven PEDV strains from Tibetan pig were 95.7%-99.9% and the deduced amino acid sequence were 93.8%-99.9%,which shared 93.1%-98.3% identity with S gene nucleotide sequence and 91.0%-98.1% identity with S gene amino acid sequence. S gene of seven strains of PEDV from Tibetan pigs formed two separate independent branches between G1 and G2 branches, indicating that they might represent two novel S genotypes. The mutation of L535 I amino acid mutation was firstly found in COE and epitope in the S protein. These results indicated that the infection rate of PEDV in Tibetan pigs in Ganzi Prefecture of Sichuan Province was high, and the S gene showed a unique genetic evolution trend.
作者
杨丹娇
张敏
蓝岚
潘瑶
李友英
吴建平
汤承
叶忠明
YANG Danjiao;ZHANG Min;LAN Lan;PAN Yao;LI Youying;WU Jianping;TANG Cheng;YE Zhongming(Institute of Animal Science of Ganzi Tibetan Autonomous Prefecture of Sichuan Province,Kangding 626000,China;College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2021年第15期17-22,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
四川省中央引导地方科技发展专项(2019ZYD084)。
关键词
甘孜州
藏猪
猪流行性腹泻病毒
S基因
遗传变异
Ganzi Prefecture
Tibetan pigs
Porcine epidemic diarrhea virus
S gene
genetic variation
