摘要
研究不同时长冷暴露对仔猪前肢骨骼肌、肺脏、心脏、肾脏、胃和十二指肠组织结构的影响。建立急性冷暴露模型,并在冷暴露周期结束后麻醉处死并采集仔猪前肢骨骼肌、肺脏、心脏、肾脏、胃和十二指肠的组织样品进行固定,采用H.E染色法制作组织切片,镜下观察。观察发现冷暴露对各组织造成的损伤程度不同,不同组织间比较,肺脏损伤较为严重;在仔猪前肢骨骼肌、肺脏、肾脏、胃和十二指肠组织中,同组织间相比冷暴露12 h造成的损伤最为严重。冷暴露后仔猪肺脏组织主要出现肺泡间质增宽等变化;仔猪前肢骨骼肌组织主要出现肌纤维间质增宽,细胞颗粒变性,明显水肿等变化;仔猪肾脏组织主要出现细胞水肿,肾小管上皮细胞坏死变性等变化;仔猪胃组织主要出现细胞坏死,胃底绒毛脱落,出血及腺体的数量和体积变少等变化;仔猪十二指肠组织主要出现绒毛坏死脱落,严重充血,炎性细胞浸润等变化。仔猪心脏组织主要出现心肌纤维断裂,水肿和充出血现象等变化,其中心脏在冷暴露2 h和6 h后组织损伤相比于对照组较为严重,而冷暴露12 h后损伤较轻。急性冷暴露造成仔猪骨骼肌、肺脏、心脏、肾脏、胃和十二指肠的组织结构形态发生改变,进而在器官水平上影响仔猪的生理功能。
To study the effects of different lengths of cold exposure on the skeletal muscle,lung,heart,kidney,stomach,and duodenal tissue structure of piglet forelimbs,an acute cold exposure model was established,and anesthetized after the end of the cold exposure cycle and collect piglet forelimb skeletal muscle,lung,heart,kidney,stomach,and duodenum tissue samples for fixation,and HE staining was used to make tissue sections,observed under the microscope.With the increase of the duration of cold exposure,the structural changes of piglet skeletal muscles,lungs,kidneys,stomach and duodenum increased accordingly.The changes of the 12-hour cold exposure group were the most obvious.After cold exposure,the lung tissue of piglets mainly showed changes such as broadening of the alveolar interstitial;the piglet forelimb skeletal muscle tissue mainly showed changes of interstitial muscle fiber,cell granule degeneration,and obvious edema;the piglet kidney tissue mainly showed cell edema and renal tubular epithelial cells Necrotic degeneration and other changes;piglet stomach tissue mainly had cell necrosis,gastric fundus villi,hemorrhage,and the number and volume of glands were reduced;piglet duodenal tissue mainly appeared villi necrosis and shedding,severe congestion,and inflammatory cell infiltration wait for changes.Piglet heart tissue mainly showed changes in myocardial fiber rupture,edema,and hyperemia.Among them,the tissue damage of the heart was more serious than that of the control group after 2 hours and 6 hours of cold exposure,and the damage was lighter after 12 hours of cold exposure.Acute cold exposure caused changes in the histological structure of piglet skeletal muscle,lung,heart,kidney,stomach and duodenum,which in turn affected the physiological function of the piglet at the organ level.
作者
徐晶
邵子益
计红
袁建彬
范春玲
蒋剑成
杨闯
甄莉
郭景茹
Xu Jing;Shao Ziyi;Ji Hong;Yuan Jianbin;Fan Chunling;Jiang Jiancheng;Yang Chuang;Zhen Li;Guo Jingru(College of Animal Science and Fechnology,Heilongjiang Bayi Agricultural University,Daqing 163319)
出处
《黑龙江八一农垦大学学报》
2021年第4期33-39,共7页
journal of heilongjiang bayi agricultural university
基金
黑龙江省自然科学基金(LH2020C081)
中国博士后科学基金资助(2017M621313)
黑龙江省博士后科学基金资助(LBH-Z17184)
黑龙江八一农垦大学三横三纵项目(ZRCQC201804)
国家自然科学基金资助项目(31502029,31372398)
中国博士后科学基金(2017M611404)
黑龙江省农垦总局项目(HKKYZD190308)。
关键词
冷暴露
仔猪
切片
组织损伤
cold exposure
piglets
section
tissue damage
