摘要
为了研究罗汉果(Siraitia grosvenorii)多糖及其羧甲基化产物的结构和抗氧化性能,使用酶解法从罗汉果中提取了罗汉果多糖,并进行了羧甲基化修饰,然后对罗汉果多糖进行了GC-MS单糖分析和傅里叶红外光谱分析,并研究罗汉果多糖及其羧甲基化产物对1,1-二苯基-2-三硝基苯肼(DPPH)和羟基自由基(·OH)的清除能力。结果表明,罗汉果多糖提取率为3.64%,羧甲基化取代度为0.41。应用GC/MS技术对罗汉果多糖进行单糖组分分析,结合面积归一法得出罗汉果多糖由呋喃糖(3.53%)、阿拉伯糖(7.22%)、甘露糖(20.60%)、葡萄糖(38.03%)、木糖(0.73%)、半乳糖(28.65%)、半乳糖醛酸(0.51%)、葡萄糖醛酸(0.74%)组成。经过抗氧化活性测试可知,与修饰前比较,羧甲基化修饰后的罗汉果多糖具有更强的抗氧化性能。
In order to study the structure and antioxidant properties of Siraitia grosvenorii polysaccharide and its carboxymethylated products,Siraitia grosvenorii polysaccharide was extracted from Siraitia grosvenorii using enzymatic hydrolysis method and modified by carboxymethylation.GC-MS monosaccharide analysis was carried out and Fourier Infrared Spectroscopy.The scavenging ability of polysaccharides of Siraitia grosvenorii and its carboxymethylation products on 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and hydroxyl radical(·OH)was also studied.The results showed that the extraction rate of Siraitia grosvenorii polysaccharide was 3.64,and the degree of carboxymethylation substitution was 0.41.GC/MS technology was used to analyze the monosaccharide composition of Siraitia grosvenorii polysaccharides.Combined with the area normalization method,Siraitia grosvenorii polysaccharides were obtained from furanose(3.53%),arabinose(7.22%),mannose(20.60%),glucose(38.03%),xylose(0.73%),galactose(28.65%),galact⁃uronic acid(0.51%),glucuronic acid(0.74%).Compared with that before modification,the polysaccharides modified by carboxy⁃methylation had stronger antioxidant properties.
作者
白家峰
姚延超
郑毅
刘绍华
刘启斌
周奕
邢雨晴
杨雯静
许春平
BAI Jia-feng;YAO Yan-chao;ZHENG Yi;LIU Shao-hua;LIU Qi-bin;ZHOU Yi;XING Yu-qing;YANG Wen-jing;XU Chun-ping(Technical Center of China Tobacco Guangxi Industrial Co.Ltd.,Nanning 530001,China;College of Food and Biology Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;Technical Center of Guangxi Zhenlong Industrial Co.Ltd.,Hezhou 542600,Guangxi,China)
出处
《湖北农业科学》
2021年第15期107-111,共5页
Hubei Agricultural Sciences
基金
国家自然科学基金联合项目(U1604176)
广西中烟合作项目(GXZYZZ2020C004)。
