摘要
目的探讨不同浓度高良姜素(Ga)对于人肺腺癌A549、PC9细胞增殖、迁移能力的影响及其可能的机制。方法体外培养肺腺癌A549、PC9细胞,并分为对照组(DMSO溶剂对照组、0药对照组)和实验组(Ga浓度分别为12.5、25、50、100μmol/L)。使用CCK-8实验和细胞划痕实验分别检测各组肺腺癌细胞的增殖和迁移能力;生化检测各组细胞乳酸(LD)生成量、乳酸脱氢酶(LDH)活性以及ATP生成量;ELISA法检测各组细胞上清液中的E-钙黏蛋白(E-cad)和N-钙黏蛋白(E-cad)的蛋白表达水平。统计分析上述各指标的组间差异。结果CCK-8实验表明,Ga作用于肺腺癌细胞后,细胞出现一定的增殖抑制现象。细胞划痕实验表明,Ga作用于肺腺癌细胞后,细胞出现一定的迁移抑制现象。生化检测实验表明,Ga作用于肺腺癌细胞后,LD生成量、LDH活性及ATP生成量均出现降低现象。ELISA法表明,Ga作用于肺腺癌细胞后,实验组细胞上清液中E-cad蛋白表达量高于对照组,而N-cad蛋白表达量低于对照组。结论Ga在体外实验中可以抑制肺腺癌A549、PC9细胞的增殖、迁移能力,并且有可能系通过抑制糖酵解及上皮间质转化(EMT)途径实现。
Objective To research the effects of different concentrations of galangin(Ga)on the proliferation and migration of the A549 and PC9 cells of human lung adenocarcinoma and their possible mechanisms.Methods A549 and PC9 cells were cultured in vitro and were divided into control group(DMSO solvent control group,0 Ga control group)and experimental groups,which contained different concentrations(12.5,25,50 and 100μmol/L),respectively.CCK-8 test and cell scratch test were used to detect the effects of the proliferation and migration of lung adenocarcinoma cells in each group.The changes of lactic acid(LD)production,lactate dehydrogenase(LDH)activity and ATP production were detected by biochemical tests.The expression of E-cadherin(E-cad)and N-cadherin(N-cad)in cells was detected by ELISA kits.Differences between groups in each index were compared by statistical analysis.Results CCK-8 results showed that Ga had an inhibitory effect on the proliferation of lung adenocarcinoma cells.Cell scratch results showed that Ga had an inhibitory effect on the migration of lung adenocarcinoma cells.Biochemical detection results showed that Ga had an inhibitory effect on LD production,LDH activity and ATP production.ELISA results showed that E-cad protein expression in the supernatant in the experimental groups was higher than that in the control group,while N-cad protein expression was lower than that in the control group.Conclusion Ga can inhibit the proliferation and migration of A549 and PC9 cells of lung adenocarcinoma in vitro,possibly by inhibiting aerobic glycolysis and epithelial-mesenchymal transition.
作者
汪新
束军
代丽丽
沈继龙
Wang Xin;Shu Jun;Dai Lili(Dept of Respiratory Medicine, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230032)
出处
《安徽医科大学学报》
CAS
北大核心
2021年第8期1212-1217,共6页
Acta Universitatis Medicinalis Anhui
基金
安徽高校自然科学研究重点项目(编号:KJ2018A0204)。
关键词
高良姜素
肺腺癌
增殖
迁移
galangin
lung adenocarcinoma
proliferation
migration
