摘要
【目的】研究武定乌骨品系鸡与非乌骨品系鸡酪氨酸酶(TYR)基因多态性及TYR表达差异与黑色素沉积的关系,探究武定鸡乌骨品系鸡乌肉性状形成的分子机理。【方法】以武定乌骨品系鸡和非乌骨品系鸡为研究对象,测定两品系300只300日龄鸡血浆中TYR活力、总黑色素含量以及血浆比色OD值,采用直接测序法分析武定鸡TYR基因外显子的多态位点(SNPs),并检测各组织中TYR基因表达差异。【结果】与武定非乌骨品系鸡相比,武定乌骨品系鸡血浆各指标均较高;在TYR外显子区域共发现2个SNP位点(C2744T和C2866T),符合哈代温伯格平衡,呈中度多态;TYR基因C2744T位点CC基因型的血浆TYR活力以及总黑色素均高于其他基因型,并呈现较低亮度值的差异;在基因表达量方面,武定乌骨品系鸡各组织的TYR基因表达量均极显著高于武定非乌骨品系鸡(P<0.01)。【结论】TYR基因C2744T位点的CC基因型可作为武定乌骨品系鸡选育的遗传标记,其他指标可作为辅助参照。
[Purpose]To study the relationship between the TYR gene polymorphism with the difference of TYR expression and melanin deposition in Wuding black-boned chicken and non-black-boned chicken.[Method]Taking Wuding black-boned chicken and non-black-boned chicken as research objects,the TYR vitality,total melanin content and plasma colorimetry(OD value)in the plasma of two strains of 300 chickens at the age of 300 days was measured.Then,direct sequencing was used to analyze the polymorphic sites(SNPs)of the TYR gene exons in Wuding chickens and to detect the differences in TYR gene expression in various tissues.[Result]Compared with Wuding non-black-boned chicken,Wuding black-boned chicken had higher plasma indexes.Two SNP sites(C2744T and C2866T)were found in the TYR exon region,which were in line with the Hardy-Weinberg equilibrium and showed moderate polymorphism.Compared with other genotypes,CC genotype of TYR locus C2744T showed higher TYR vitality and total melanin than other genotypes,but lower luminance value.In terms of gene expression level,TYR gene expression level in various tissues of Wuding black-boned chicken was significantly higher than that of non-black-boned chicken(P<0.01).[Conclusion]CC genotype at C2744T of TYR gene can be used as genetic marker for chicken breeding of Wuding black-boned chicken,and other indicators can be used as auxiliary reference.
作者
徐志强
李丰耘
赵净颖
豆腾飞
王坤
黄英
葛长荣
贾俊静
XU Zhiqiang;LI Fengyun;ZHAO Jingying;DOU Tengfei;WANG Kun;HUANG Ying;GE Changrong;JIA Junjing(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第4期608-615,共8页
Journal of Yunnan Agricultural University:Natural Science
基金
云南农业大学畜禽健康养殖与品质安全省创新团队(2019HC011)
云岭学者(云组通[2014]48号)
云岭产业技术领军人才(云发改人事[2015]1495号)。
