摘要
以高山植物圆锥南芥和其近缘物种拟南芥种子为研究材料,通过在生长培养基中添加不同浓度的甘露醇(150、300和600 mmol/L)模拟干旱胁迫,比较两物种的萌发情况,并检测甘露醇处理前后两种植物种子中的内源激素脱落酸(ABA)含量、热激蛋白HSP101和HSP70的表达量,研究圆锥南芥适应高山干旱环境的可能机制。结果显示:圆锥南芥种子对甘露醇更敏感,当甘露醇浓度超过300 mmol/L时,出现暂时休眠的现象;正常情况下,圆锥南芥种子中ABA含量显著高于拟南芥;甘露醇处理后,两物种ABA含量都有所上升,圆锥南芥ABA含量显著高于拟南芥;正常情况下,两物种的HSP101和HSP70表达量都较低;甘露醇处理后,拟南芥的HSP101和HSP70的表达量明显增强,而圆锥南芥的HSP表达量不受影响。研究表明,圆锥南芥在干旱胁迫下保持高含量ABA以启动种子休眠,推测其不合成HSP蛋白等生物大分子物质是一种节约能量的方式,这是圆锥南芥对高山干旱环境的适应性策略。
The seeds of Arabis paniculata and its relative Arabidopsis thaliana were used as materials in the present study.The germination rate,the content of ABA and the accumulation of HSP101 and HSP70 were compared in these two species before and after treatment of mannitol with different concentrations(150,300 and 600 mmol/L).The adaptation mechanism of A.paniculata to alpine environment was discussed.The results were presented as following:the seeds of A.paniculata were more sensitive to the treatment of mannitol;temporary seed dormancy was exhibited in A.paniculata when the concentration of mannitol exceeded 300 mmol/L;the ABA content of the seeds in A.paniculata was significantly higher than that in A.thaliana under the normal growth conditions and mannitol treatment.The abundance of HSP101 and HSP70 was low in both species under normal growth conditions.The protein levels of HSP101 and HSP70 were increased rapidly under mannitol treatment in A.thaliana,while those were still kept low in A.paniculata.These results indicated that seed dormancy was induced in A.paniculata upon high accumulation of ABA to adaptation to alpine environment with limited water in winter.A energy conservation strategy was speculated according to the fact that the protein levels of HSP101 and HSP70 were not affected in A.paniculata under drought stress.
作者
唐婷
陶发清
李唯奇
TANG Ting;TAO Faqing;LI Weiqi(School of Life Sciences,Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization,Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils,Hunan University of Science and Technology,Xiangtan 411201,China;Plant Germplasm and Genomics Center,Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2021年第4期92-95,共4页
Journal of Biology
基金
湖南省教育厅科学研究项目(18B215)。
