摘要
为了解杜鹃光合机构对高温的响应机制与筛选耐热品种,以7个杜鹃品种‘胭脂蜜’、‘吐蕊玫瑰’、‘小青莲’、‘夏锦袍’、‘锦袍’、‘艳春’与‘紫鹤’为材料,研究了3种高温(30℃/28℃、35℃/30℃、38℃/35℃)胁迫下,供试杜鹃品种的外观形态与叶绿素荧光参数Fo(初始荧光)、Fv/Fo(PSII潜在活性)、Fv/Fm(最大光化学效率)、Fv’/Fm’(PSII反应中心激发能捕获效率)与NPQ(非光化学猝灭系数)的变化。结果表明:供试杜鹃品种随着胁迫时间增加Fo上升,Fv/Fo、Fv/Fm与Fv’/Fm’下降,变化幅度与胁迫强度及时间成正比;NPQ表现为前期下降后期升高,耐热性强的杜鹃品种叶绿素荧光参数变化幅度小而耐热性差的品种变化幅度大,与外观表现一致。隶属函数综合分析表明,‘胭脂蜜’、‘吐蕊玫瑰’、‘小青莲’耐热性较强,可部分替代常规品种‘紫鹤’应用于城市绿化。
In order to understand the response mechanism of Rhododendron to high temperature stress andselect cultivars with high heat- tolerance, appearance and chlorophyll fluorescence were investigated in R.obtusum‘Yanzhimi＇, R. obtusum‘Turuimeigui＇, R. obtusum‘Xiaoqinglian＇, R. pulchrum‘Xiajinpao＇, R.hybridum‘Jinpao＇, R. hybridum‘Yanchun＇and R. Championiae‘Zihe＇under three high- temperaturestresses（30℃/28℃, 35℃/30℃ and 38℃/35℃）. The chlorophyll fluorescence indexes included Fo（initialfluorescence）, Fv/Fo（potential activity of PSII）, Fv＇/Fm＇（efficiency of excitation capture of open PSII center）and NPQ（non- photochemical quenching）. The results indicated that with the increase of treated days, Fo increased, Fv/Fo and Fv＇/Fm＇decreased. The fluorescence change was proportional to the stress strength andtreated days. Moreover, NPQ reduced in the early stage and then increased. The change range of chlorophyllfluorescence was small in cultivars with high heat- tolerance while that was large in those with low heat-tolerance. The heat- tolerance of tested Rhododendrons was comprehensively evaluated with subordinatefunction. The cultivars with higher heat-tolerance were‘Yanzhimi＇,‘Turuimeigui＇and‘Xiaoqinglian＇, andwhich could be applied to city landscape and partly substitute conventional cultivar‘Zihe＇.
出处
《中国农学通报》
2015年第31期150-159,共10页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金青年科学基金项目"不同基因型杜鹃在干旱胁迫下的生理响应与细胞程序性死亡研究"(31300587)
武汉市农科院2015年科技创新项目"特色花果种质资源收集
筛选与新品种培育技术研究"(Cxtd201506)
关键词
杜鹃
高温胁迫
耐热性
叶绿素荧光
隶属函数
Rhododendron
high temperature stress
heat tolerance
chlorophyll fluorescence
subordinate function
