摘要
目的:观察平喘颗粒对哮喘小鼠肺组织中Ⅱ型固有淋巴细胞(ILC2)数量和巨噬细胞M2型极化的影响。方法:采用卵清蛋白致敏和激发构建哮喘小鼠模型,在此基础上分别给予平喘颗粒或IL⁃33中和抗体进行干预,分别通过HE、PAS和Masson染色观察各组小鼠肺组织病理形态,分别以ELISA和qRT⁃PCR法检测肺泡灌洗液及肺组织中IL⁃4、IL⁃5、IL⁃13和IL⁃33的表达,采用流式细胞术检测肺组织中Ⅱ型固有淋巴细胞和M2型巨噬细胞的数量,采用Westernblot法检测肺组织中ST⁃2、FIZZ1和Arg⁃1蛋白的表达水平。结果:与空白组相比,模型组小鼠肺组织炎症评分、PAS评分和胶原染色面积明显增加,肺泡灌洗液和肺组织中IL⁃4、IL⁃5、IL⁃13和IL⁃33的表达明显增加,肺组织中ILC2和M2型巨噬细胞的数量、ST⁃2、FIZZ1和Arg⁃1蛋白的表达明显增加,差异均具有统计学意义(P<0.05);与模型组相比,平喘颗粒能够降低哮喘小鼠肺组织炎症评分、PAS评分和胶原染色面积,下调肺泡灌洗液和肺组织中IL⁃4、IL⁃5、IL⁃13和IL⁃33的表达,降低肺组织中ILC2和M2型巨噬细胞的数量、ST⁃2、FIZZ1和Arg⁃1蛋白的表达,差异均具有统计学意义(P<0.05);结论:平喘颗粒通过抑制ILC2介导的M2型巨噬细胞极化,从而改善哮喘气道重塑,达到治疗哮喘的作用。
Objective:To observe the effect of Pingchuan granule on the number of typeⅡinnate lymphocytes(ILC2)and M2 polarization of macrophages in the lung tissue of asthmatic mice.Methods:Ovalbumin sensitized and challenged asthmatic mouse models were established,and then Pingchuan granules or IL⁃33 neutralizing antibody were given to intervene.The patho⁃logical morphology of lung tissue was observed by HE,PAS and Masson staining,and the expressions of IL⁃4,IL⁃5,IL⁃13 and IL⁃33 in BALF and lung tissue were detected by ELISA and qRT⁃PCR.Flow cytometry was used to detect the number of type II innate lymphocytes and type M2 macrophages in lung tissue.Western blot was used to detect the protein expression levels of ST⁃2,FIZZ1 and Arg⁃1 in lung tissue.Results:Compared with the control group,the inflammation score,PAS score and colla⁃gen staining area of the model group were significantly increased,the expressions of IL⁃4,IL⁃5,IL⁃13 and IL⁃33 in BALF and lung tissue were significantly increased,the number of ILC2 and M2 macrophages,the expression of ST⁃2,FIZZ1 and Arg⁃1 pro⁃tein in lung tissue were significantly increased,and the differences were statistically significant(P<0.05);Compared with the model group,Pingchuan granule could significantly reduce the inflammation score,PAS score and collagen staining area of asth⁃matic mice,down⁃regulate the expression of IL⁃4,IL⁃5,IL⁃13 and IL⁃33 in BALF and lung tissue,reduce the number of ILC2 and M2 macrophages,and the expression of ST⁃2,FIZZ1 and Arg⁃1 protein in lung tissue(P<0.05).Conclusion:Pingchuan granule improve the airway remodeling of asthma by inhibiting the polarization of M2 macrophages mediated by ILC2.
作者
王瑶
袁星星
田春燕
李竹英
WANG Yao;YUAN Xing‑xing;TIAN Chun‑yan;LI Zhu‑ying(Heilongjiang University of Chinese Medicine,Harbin 150040,China;the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Nangang Branch of Heilongjiang Academy of Traditional Chinese Medicine,Harbin 150006,China)
出处
《海南医学院学报》
CAS
2021年第16期1201-1206,1212,共7页
Journal of Hainan Medical University
基金
国家自然科学基金面上项目(82074365)
黑龙江省自然科学基金面上项目(H2017068)
哈尔滨市应用技术研究与开发项目(2017RAXXJ053)。
