摘要
海马组织在学习、记忆中发挥着重要作用,而且海马组织对巨细胞病毒(Cytomegalovirus,CMV)感染非常敏感,会使海马组织中的神经元大量丢失,神经元细胞发生凋亡。芍药苷可以减轻小鼠炎性脑损伤,其可能与提高机体抗氧化能力、清除体内自由基有关,但是芍药苷在CMV感染小鼠中研究甚少。为探讨芍药苷对CMV感染小鼠脑组织损伤及海马神经元细胞凋亡的影响,本研究将新生昆明小鼠随机分为4组:对照组、小鼠巨细胞病毒组(MCMV组)、芍药苷低剂量组(Pae-L组)和芍药苷高剂量组(Pae-H组),后三组通过腹腔注射MCMV病毒悬液建立模型,之后对照组和MCMV组经腹腔注射生理盐水,Pae-L组经腹腔注射10 mg/kg芍药苷,Pae-H组经腹腔注射30 mg/kg芍药苷;荧光定量PCR检测各组小鼠脑组织中MCMV-DNA载量的变化;Morris水迷宫检测各组小鼠学习记忆功能指标的变化;蛋白质免疫印迹(Western Blot)检测各组小鼠脑组织中MMP-9的表达变化;NeuN免疫染色检测各组小鼠海马组织中NeuN阳性细胞的变化;TUNEL染色检测各组小鼠海马组织中神经元细胞凋亡的变化;Western Blot检测各组小鼠海马组织中Bax、Bcl-2蛋白表达的变化。结果显示,与对照组比较,MCMV组小鼠的逃避潜伏期明显增加,穿越平台次数明显减少,脑组织中MMP-9蛋白表达水平显著上调,海马组织中NeuN阳性细胞数目显著减少,海马组织中神经元细胞凋亡指数显著增加,海马组织中Bax蛋白表达水平显著上调,Bcl-2蛋白表达水平显著下调;与MCMV组比较,Pae-L组和Pae-H组小鼠脑组织中MCMV-DNA载量减少,逃避潜伏期减少,穿越平台次数增加,脑组织中MMP-9蛋白表达水平下调,海马组织中NeuN阳性细胞数目增加,海马组织中神经元细胞凋亡指数减少,海马组织中Bax蛋白表达水平下调,Bcl-2蛋白表达水平明显上调。本研究提示,芍药苷能够改善MCMV感染引起的脑损伤,减轻海马组织中神经元细
Hippocampus plays an important role in learning and memory,and the hippocampus is very sensitive to cytomegalovirus(CMV)infection,which will cause a large number of neurons in hippocampus to lose and apoptosis.Paeoniflorin can reduce inflammatory brain injury in mice,which may be related to improving the antioxidant capacity of the body and clearing free radicals in vivo.However,paeoniflorin has been rarely studied in mice infected with CMV.In order to explore the effects of paeoniflorin on brain tissue damage and hippocampal neuronal cell apoptosis in mice infected with CMV,newborn Kunming mice were randomly divided into four groups:control(control),mouse cytomegalovirus(MCMV),paeoniflorin low⁃dose(Pae⁃L)and paeoniflorin high⁃dose(Pae⁃H).The latter three groups were injected with MCMV virus suspension into the abdominal cavity to establish models,the control and MCMV groups were intraperitoneally injected with normal saline,the Pae⁃L group was intraperitoneally injected with 10 mg/kg paeoniflorin,and the Pae⁃H group was intraperitoneally injected with 30 mg/kg paeoniflorin.Fluorescence quantitative PCR was used to detect changes in MCMV⁃DNA load in the brain tissue.The Morris water maze was used to measure learning and memory function indices.Western Blot was performed to measure expression changes in MMP⁃9 in brain tissue.NeuN immunostaining was used to detect changes in NeuN⁃positive cells in the hippocampus.TUNEL staining was used to detect changes in neuronal cell apoptosis in the hippocampus.Western Blot was used to measure changes in Bax and Bcl⁃2 protein expression in the hippocampus.Results showed that compared with the control group,escape latency was significantly increased,the number of crossing platforms was significantly reduced,and the expression level of MMP⁃9 protein in brain tissue was significantly up⁃regulated in treated groups.Additionally,the number of NeuN⁃positive cells in hippocampus tissue was significantly reduced,the neuronal apoptosis index in hippocampus tis
作者
骆剑蛟
刘亚茹
陈文
LUO Jianjiao;LIU Yaru;CHEN Wen(Pharmacy Department,Traditional Chinese Medicine of Jingdezhen,Jingdezhen 333000,China;Science and Education Department,Traditional Chinese Medicine of Jingdezhen,Jingdezhen 333000,China;Jiangxi College of Traditional Chinese Medicine,Fuzhou 333000,China)
出处
《病毒学报》
CAS
CSCD
北大核心
2021年第4期790-797,共8页
Chinese Journal of Virology
