摘要
目的观察胸腔热灌注(IHP)对兔急性肺损伤(ALI)中Toll样受体4(TLR4)/核转录因子(NF-κB)/p38丝裂原活化蛋白激酶(p38MAPK)炎症信号通路的影响。方法新西兰大白兔31只,按随机数字表法分为4组,假撞击组(A组,7只)、胸部撞击组(B组,8只)、撞击+35℃常温灌注组(C组,8只)和撞击+43℃度热灌注组(D组,8只),垂直撞击兔右侧胸部,建立ALI模型行IHP,酶联免疫吸附试验(ELISA)法检测肺组织中热休克蛋白70(HSP70)及肺泡灌洗液中白细胞介素(IL)-1、IL-6、肿瘤坏死因子(TNF-α)的含量。荧光定量PCR技术检测肺组织中NF-κB、p38MAPK、TLR4。蛋白质印迹法(Western blot)检测肺组织p-ATF2、磷酸化蛋白p38(p-p38)蛋白表达情况,并进行组间比较,组间比较采用t检验。结果IHP12 h后,D组的HSP70(17.40±2.30)高于A组(5.30±2.40)、B组(6.10±2.10)、C组(6.20±2.60),差异有统计学意义(t=9.126、10.262、9.963,P<0.05)。D组NF-κB的mRNA(2.74±0.10)高于A组(0.73±0.13)、B组(1.85±0.07)、C组(1.41±0.28),差异有统计学意义(t=33.823、20.623、12.652,P<0.05)。D组p38MAPK的mRNA(2.49±0.23)高于A组(0.39±0.12)、B组(1.43±0.16)、C组(1.25±0.08),差异有统计学意义(t=21.648、10.701、14.403,P<0.05)。D组TLR4的mRNA(3.41±0.32)高于A组(0.56±0.18)、B组(2.32±0.18)、C组(1.73±0.11),差异有统计学意义(t=22.347、8.397、14.043,P<0.05)。D组磷酸化活化转录因子2(p-ATF2)的蛋白灰度值(1.04±0.12)高于A组(0.48±0.11)、C组(0.65±0.08),差异有统计学意义(t=9.369、7.649,P<0.05)。D组p-p38的蛋白灰度值(0.79±0.13)高于A组(0.37±0.09)、C组(0.53±0.07),差异有统计学意义(t=7.162、4.981,P<0.05)。D组的TNF-α(50.30±7.70)低于B组(94.40±5.80)和C组(79.60±8.40),差异有统计学意义(t=-12.939、-7.273,P<0.05)。D组的IL-1(63.40±9.70)低于B组(135.70±6.80)和C组(103.20±8.40),差异有统计学意义(t=-17.263、-8.773,P<0.05)。D组的IL-6(34.20±3.70)低于B组(78.40±2.90)和C组(56.70±2.80),差异有统计�
Objective To observe the effect of pleural intrapleural hyperthermic perfusion(IHP)on inflammatory signal pathway toll like receptor 4(TLR4)/nuclear transcription(NF-κB)/p38 mitogen-activated protein kinase(p38MAPK)in rabbits with acute lung injury(ALI).Methods Totally,31 New Zealand white rabbits were randomly divided into sham impact group(A,n=7),chest impact group(B,n=8),impact+35℃lavage group(C,n=8)and impact+43℃ heat perfusion group(D,n=8).The rabbit model of ALI was established.The levels of heat shock protein 70(HSP70)in lung tissue and interleukin(IL)-1,IL-6 and tumor necrosis factor-α(TNF-α)in bronchoalveolar lavage fluid(BALF)were measured by enzyme linked immunosorbent assay(ELISA).TLR4/NF-κB/p38MAPK in lung tissue was detected by quantitative real-time PCR.The protein expression of p-ATF2 and p-p38 in lung tissues was detected by Western blotting and compared between groups.Results After 12 h of IHP,the HSP70(17.40±2.30)in group D was significantly higher than that in group A(5.30±2.40),group B(6.10±2.10),and group C(6.20±2.60).The difference was statistically significant(t=9.126,10.262,9.963,P<0.05).The mRNA expression of NF-κB in group D(2.74±0.10)was significantly higher than that in group A(0.73±0.13),group B(1.85±0.07),and group C(1.41±0.28,t=33.823,20.623,12.652,P<0.05).The mRNA expression of p38MAPK in group D(2.49±0.23)was significantly higher than that in group A(0.39±0.12),group B(1.43±0.16),and group C(1.25±0.08,t=21.648,10.701,14.403,P<0.05).The mRNA expression of TLR4 in group D(3.41±0.32)was significantly higher than that in group A(0.56±0.18),group B(2.32±0.18),and group C(1.73±0.11,t=22.347,8.397,14.043,P<0.05).The protein gray value of p-ATF2 in group D(1.04±0.12)was significantly higher than that in group A(0.48±0.11)and group C(0.65±0.08,t=9.369,7.649,P<0.05).The protein gray value of p-p38 in group D(0.79±0.13)was significantly higher than that in group A(0.37±0.09)and group C(0.53±0.07,t=7.162,4.981,P<0.05).The TNF-α in group D(50.30±7.70)was signi
作者
王立
张力为
尚文军
陈忠杰
丁东晓
应俊杰
何毛伟
Wang Li;Zhang Liwei;Shang Wenjun;Ding Dongxiao;Ying Junjie;Chen Zhongjie;He Maowei(Department of Thoracic Surgery,Beilun District People's Hospital,Ningbo 315800,China;Department of Thoracic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)
出处
《中华实验外科杂志》
CAS
北大核心
2021年第8期1447-1450,共4页
Chinese Journal of Experimental Surgery
基金
宁波市自然科学基金(2019A610236)。
