摘要
旨为核桃细菌性黑斑病天然杀菌剂的开发提供理论依据,同时为白藜芦醇在农林作物病害防治上的应用奠定基础。测定了白藜芦醇对核桃细菌性黑斑病菌(Xaj)的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。在白藜芦醇的亚抑菌浓度下,开展了以下实验:(1)通过结晶紫染色法检测了Xaj生物膜的形成;(2)测定了胞外多糖的产生量;(3)用生物化学方法在相应的固体培养基上分析了Xaj胞外酶的分泌情况;(4)在swarming培养基上检测了Xaj的运动能力。结果显示,白藜芦醇对Xaj的MIC为20μg/mL,MBC为45μg/mL。在亚抑菌浓度为15μg/mL的条件下,白藜芦醇能够显著抑制Xaj生物被膜的形成;对该菌EPS的产生也有明显抑制作用;能显著抑制Xaj蛋白酶的分泌,对淀粉酶的分泌有促进作用,对纤维素酶的分泌没有影响;对Xaj运动性没有影响。白藜芦醇对核桃黑斑病菌有抑制作用,影响该菌的毒力因子,有望为核桃黑斑病的防治开发出合适的植物源杀菌剂。
It aims to provide theoretical basis for the development of natural bactericide for walnut bacterial black spot,and to lay a foundation for the application of resveratrol in the prevention and control of agricultural and forestry crop diseases.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of resveratrol against Xanthomonas arboricola pv.juglandis(Xaj)were determined.The following experiments were carried out under the sub-inhibitory concentration of resveratrol:1)The formation of Xaj biofilm was detected by crystal violet staining.2)The production of exopolysaccharide(EPS)was determined.3)The secretion of extracellular enzyme was analyzed on the corresponding solid medium by biochemical method.4)The motility of Xaj was detected on swarming medium.The results revealed that the MIC of resveratrol against Xaj was 20μg/mL,and MBC was 45μg/mL.The formation of Xaj biofilm was effectively inhibited by resveratrol at a sub-inhibitory concentration of 15μg/mL.Under this concentration,the production of EPS was also significantly inhibited.Resveratrol effectively inhibited the secretion of Xaj protease,promoted the secretion of amylase,but had no effect on the secretion of cellulase.There was no effect on motility(swarming).Resveratrol has an inhibitory effect on walnut bacterial black spot pathogen,affecting the virulence factors of this bacterium,and is expected to develop a suitable plant-derived fungicide for the prevention and treatment of walnut black spot disease.
作者
李峰
陈雯雯
邓江丽
毛清黎
权文利
毛雅慧
LI Feng;CHEN Wen-wen;DENG Jiang-li;MAO Qing-li;QUAN Wen-li;MAO Ya-hui(Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables/College of Life Science and Technology,Hubei Engineering University,Xiaogan 432000;College of Food Science and Technology,Wuhan University of Bioengineering,Wuhan 430415)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第6期58-65,共8页
Biotechnology Bulletin
基金
国家自然科学基金项目(31700567,31370691)
2019年中央引导地方科技发展专项(2019ZYYD045)。
关键词
白藜芦醇
核桃细菌性黑斑病菌
抑菌效果
植物源杀菌剂
resveratrol
walnut bacterial black spot pathogen
antibacterial effect
botanical fungicides
