摘要
目的建立稳定、成功率高的显微镜下大鼠70%肝切除+肝双重动脉血供的动物模型。方法纳入研究大鼠76只,分训练组40只、造模组36只。训练组模拟手术操作,优化围术期管理;模型组行显微镜下制备大鼠70%肝切除(肝左叶和中叶)+肝双重动脉血供手术,术后观察大鼠一般情况,计算造模成功率和1周存活率。结果通过训练组模拟操作,渡过学习曲线。造模组手术时间为(65.3±6.56)min,血管吻合时间(11.1±2.53)min。术中无明显副损伤,出血量(0.6±0.21)mL。1周后造模成功率88.9%(32/36);造模成功,大鼠术后状态良好。结论显微镜下制备大鼠70%肝切除+LDABS模型操作较为复杂,但通过一定时间显微技能训练后,通过对术式,特别是血管吻合及围手术期护理的改良及创新,使得该模型成功率高、可重复性强,为进一步研究其在肝再生、急性肝功能衰竭和肝移植等中应用奠定基础。
Objective An animal model of 70%partial hepatectomy+liver dual arterial blood supply(LDABS)was established.Methods Seventy-six rats were divided into a training group(n=40)and a model group(n=36).In the model group,70%partial hepatectomy(left lobe and middle lobe of liver)+double artery blood supply surgery of the liver was performed under a microscope.The general status of rats was observed after surgery,and the success and 1 week survival rates were calculated.Results Through simulated surgery in the training group,the learning curve was crossed.The surgery duration of the model group was(65.3±6.56)min,and the vascular anastomosis time was(11.1±2.53)min.During the surgery,there were no obvious side injuries and less bleeding,of no more than 0.8 mL,was observed.One week later,the modeling success rate was 88.9%(32/36),and the rats were in good condition.Conclusions Surgery consisting of 70%hepatectomy+LDABS in rats under a microscope is relatively complicated.However,after a period of microsurgery training,the improvements and innovation of the surgical mode,especially vascular anastomosis and perioperative nursing,resultsed in a high success rate and strong repeatability of the model,laying the foundation for further research on its application in liver regeneration,acute liver failure,and liver transplantation.
作者
田全发
郑卫华
王子航
李朋飞
张俊晶
TIAN Quanfa;ZHENG Weihua;WANG Zihang;LI Pengfei;ZHANG Junjing(Hepatobiliary Surgery,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010030,China;Andrology of Urology,Fenyang Hospital of Shanxi Province,Fenyang 032200;Hepatobiliary Surgery,Huhhot First Hospital,Hohhot 010030)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第7期15-20,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金(81560113)
内蒙古自然科学基金重大项目(2015ZD(LH)01)
内蒙古自然科学基金(2014MS0850)
内蒙古自治区高等学校青年科技领军人才支持计划(NJYT-17-A15)。
关键词
门静脉动脉化
肝双重动脉血供
围手术期
血管吻合
肝切除
portal vein arterialization
liver double artery blood supply
perioperative period
vascular anastomosis
partial hepatectomy
