摘要
A mirror-image protein-based information barcoding and storage technology wherein D-amino acids are used to encode information into mirror-image proteins that are chemically synthesized is described.These mirror-image proteins were then fused into various materials from which information-encoded objects were produced.Subsequently,the mirror-image proteins were extracted from the objects using biotin-streptavidin resin-mediated specific enrichment and cleaved using an Ni(Ⅱ)-mediated selective peptide cleavage.Protein sequencing was accomplished using liquid chromatography/tandem mass spectrometry(LC-MS/MS)and then transcoded into the recorded information.We demonstrated the use of this technology to encode Chinese words into mirror-image proteins,which were then fused onto a poly(ethylene terephthalate)(PET)film and retrieved and decoded by LC-MS/MS sequencing.Compared to information barcoding and storage technologies using natural biopolymers,the mirrorimage biopolymers used in our technology may be more stable and durable.
生物大分子用于信息存储和编码近年来受到广泛关注.本文报道了一种基于镜像蛋白的信息编码与存储方法.该方法以D-氨基酸作为信息编码与存储的基本结构单元,利用蛋白化学合成技术得到含编码信息镜像蛋白材料,并通过物理掺杂等方式将信息存储于目标物件中;通过生物素-链霉亲和素树脂介导的特异性富集从目标物件中提取镜像蛋白,并使用Ni(Ⅱ)介导的镜像蛋白序列选择性切割得到信息编码的多肽;通过LC-MS/MS测序技术实现了信息解析,最后转码还原为原始信息.使用镜像蛋白信息编码与存储技术,本文将多个汉字编码为镜像蛋白,存储至聚对苯二甲酸乙二酯(PET)薄膜中,并通过镜像蛋白信息材料富集提取、LC-MS/MS测序等操作,最后解码得到原始信息.相比于天然生物大分子作为信息编码与存储材料,本文报道的镜像蛋白具有稳定性好、持久耐用等优点.
作者
Ji-Shen Zheng
Jun Liang
Wei-Wei Shi
Ying Li
Hong-Gang Hu
Chang-Lin Tian
Lei Liu
郑基深;梁军;石威巍;李影;胡宏岗;田长麟;刘磊(Tsinghua-Peking Joint Center for Life Sciences,Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology,Center for Synthetic and Systems Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China;Hefei National Laboratory for Physical Sciences at the Microscale,School of Life Sciences,University of Science and Technology of China,Hefei 230027,China;Institute of Translational Medicine,Shanghai University,Shanghai 200444,China)
基金
the National Key R&D Program of China(2017YFA0505200 and 2019YFA0706902)
the National Natural Science Foundation of China(22022703,91753205,and 21750005)
the Science and Technological Fund of Anhui Province for Outstanding Youth(1808085J04)。
