摘要
以贵州水城小黄姜为原料,采用超声辅助提取其总黄酮,利用L9(34)正交设计进行工艺优选,并对其总黄酮提取液进行亚硝基、DPPH自由基、羟基自由基和ABTS自由基的清除活性检测。结果表明:当乙醇浓度90%、料液比1∶15、超声提取40 min时,其总黄酮的得率达到最大为1.007%,其抗氧化活性均高于50%。
Total flavonoids from Globba racemosa Smith(producing area in Shuicheng)were extracted by ultrasound-assisted method,and the process was optimized by L9(34)orthogonal design.The results showed that when the ethanol concentration was 90%,the solid to liquid ratio was 1:15,and ultrasonic extraction was 40min,the maximum yield of total flavonoids was 1.007%.The scavenging rate of nitroso,DPPH free radical,hydroxyl free radical,and ABTS free radical were detected,and it was found that their antioxidant activities were all higher than 50%.
作者
翁贵英
李金辉
朱淼
谢斐
Weng Guiying;Li Jinhui;Zhu Miao;Xie Fei(School of Biological Science and Technology,Liupanshui Normal University,Liupanshui 553001,China;School of Chemistry and Materials Engineering,Liupanshui Normal University,Liupanshui 553001,China)
出处
《六盘水师范学院学报》
2021年第3期106-111,共6页
Journal of Liupanshui Normal University
基金
2017年度贵州省特色农业种质资源开发与利用特色重点实验室(黔教合KY字2017012)
2016年度六盘水师范学院科技创新团队项目“分子生物学与生物化学科技创新团队”(LPSSYKJTD201602)。
关键词
水城小黄姜
总黄酮
抗氧化活性
Globba racemosa Smith in Shuicheng
Total Flavonoids
Antioxidant Activity
