摘要
目的探究甲状腺癌组织中长链非编码RNA(lncRNA)浆细胞瘤变体易位1(PVT1)和微小RNA(miRNA)-195-5p表达及临床意义。方法荧光实时定量PCR(qRT-PCR)技术检测江苏省常州市中医医院2017年4月至2020年1月收治的82例甲状腺癌组织、癌旁正常组织及82例甲状腺良性肿瘤组织中lncRNA PVT1和miR-195-5p的表达,分析这两个指标与甲状腺癌患者临床病理参数的关系及其相关性。结果甲状腺癌组织中lncRNA PVT1相对表达量高于甲状腺良性肿瘤组织及癌旁正常组织、而miR-195-5p相对表达量低于甲状腺良性肿瘤组织及癌旁正常组织(均P <0.05)。甲状腺癌组织中,有淋巴结转移、TNM分期Ⅲ~Ⅳ期的lncRNA PVT1相对表达量高于无淋巴结转移及Ⅰ~Ⅱ期;有淋巴结转移、TNM分期Ⅲ~Ⅳ期的miR-195-5p相对表达量低于无淋巴结转移及Ⅰ~Ⅱ期(均P <0.05);甲状腺癌组织中lncRNA PVT1表达与miR-195-5p表达呈负相关(r=-0.731,P <0.01)。结论甲状腺癌组织中lncRNA PVT1表达上调,而miR-195-5p表达下调,二者均与淋巴结转移和TNM分期有关,对评估患者病情及确定治疗靶点具有潜在的应用价值。
Objective To investigate the expression and clinical significance of long non-coding RNA(lncRNA)plasmacytoma variant translocation 1(PVT1)and microRNA(miRNA)-195-5p in thyroid carcinoma tissue.Methods The expression of lncRNA PVT1 and miR-195-5p in 82 cases of thyroid cancer tissues,adjacent normal tissues and 82 cases of benign thyroid tumor tissues treated in Changzhou of Hospital Traditional Chinese Medicine from April 2017 to January 2020 were detected by fluorescence real-time quantitative PCR(qRT-PCR),the relationship between the two indexes and clinicopathological parameters of patients with thyroid cancer and the correlation between the two inedxes was analyzed.Results The relative expression level of lncRNA PVT1 in thyroid cancer tissues was higher than that in benign thyroid tumor tissues and paracancerous normal tissues,while the relative expression level of miR-195-5p was lower than that in benign thyroid tumor tissues and paracancerous normal tissues(all P<0.05).In thyroid cancer,the relative expression level of lncRNA PVT1 with lymph node metastasis and TNM stageⅢ-Ⅳstage was higher than that without lymph node metastasis andⅠ-Ⅱstage.The relative expression level of miR-195-5p in lymph node metastasis and TNM stageⅢ-Ⅳstage was lower than that in the non-lymph node metastasis andⅠ-Ⅱstage(all P<0.05).The expression of lncRNA PVT1 in thyroid cancer tissues was negatively correlated with the expression of miR-195-5p(r=-0.731,P<0.01).Conclusion The increased lncRNA PVT1 and decreased miR-195-5p in thyroid carcinoma tissues are related to lymph node metastasis and TNM stage,which have potential application value for assessment of patients’condition and determining therapeutic targets.
作者
李小玲
邵馨
郭玲
杨亚丽
LI Xiaoling;SHAO Xin;GUO Ling;YANG Yali(Department of Pathology,Changzhou Hospital of Traditional Chinese Medicine,Jiangsu Province,Changzhou 213000,China;Department of Pathology,Qidong Hospital Affiliated to Nantong University,Jiangsu Province,Qidong 226200,China;Department of Pathology,the Second People’s Hospital of Yunnan Province,Yunnan Province,Kunming 650021,China)
出处
《中国医药导报》
CAS
2021年第19期22-26,共5页
China Medical Herald
基金
江苏省卫生和计划生育委员会科研课题(H2017092)。
