摘要
将10只雄性KM小鼠随机分为正常组及铁皮石斛多糖组,铁皮石斛多糖组小鼠按临床等效剂量(350mg/kg)灌胃8周,每天1次,每次每只0.4 mL,正常组小鼠灌胃等体积无菌水,灌胃结束后取肠道内容物及黏膜进行16S rRNA基因测序并进行功能预测,探讨铁皮石斛多糖对正常小鼠肠道菌群的影响。结果表明:铁皮石斛多糖提高了肠腔菌群的α多样性,并显著提高了肠黏膜菌群的α多样性,但β多样性的变化无统计学意义;铁皮石斛多糖使肠腔中Diaphorobacter,肠黏膜中Muribaculaceae、Staphylococcus的相对丰度显著增加,并增加了Akkermansia、Phascolarctobacterium、Turicibacter、Faecalibacteriu、Parabacteroides、Parasutterella等短链脂肪酸产生菌及Candidatus_Arthromitus、Bifidobacterium及Prevotella_9等有益菌的相对丰度;铁皮石斛多糖使小鼠肠腔及肠黏膜菌群中辅助因子与维生素代谢和氨基酸代谢的相对丰度提高。
10 male KM mice were divided into normal group and Dendrobium officinale polysaccharides(DOP)treated group.Mice in the treated group were gavaged with DOP at the clinical equivalent dose(350 mg/kg)for 8 weeks,once a day,0.4 mL each rat at a time,and the control group was gavaged with sterile water.To investigate the effect of DOP on gut microbiota of mice,the intestinal contents and mucosa were collected for 16 S rRNA gene sequencing and function prediction.The results showed that DOP increasedαdiversity of lumen-associated microbiota,especially mucosa-associated microbiota,but there was no significant difference inβdiversity.DOP significantly increased the abundance of Diaphorobacter in intestinal lumen and Muribaculaceae,Staphylococcus in intestinal mucosa.The relative abundance of short-chain fatty acids producing bacteria including Akkermansia,Phascolarctobacterium,Turicibacter,Faecalibacteriu,Parabacteroides,Parasutterella and beneficial bacteria such as CandidatusArthromitus,Bifidobacterium and Prevotella9 were increased.In addition,DOP increased metabolism pathway of cofactors,vitamins and amino acid in intestinal lumen and mucosa.
作者
谢果珍
唐圆
宁晓妹
邱集慧
谭周进
XIE Guozhen;TANG Yuan;NING Xiaomei;QIU Jihui;TAN Zhoujin(College of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Xiangxing College,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;College of Medicine,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第4期449-454,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家级大学生创新创业训练计划项目(201910541033)
湖南中医药大学研究生培养质量工程项目(2019CX08)。
关键词
小鼠
铁皮石斛多糖
肠腔菌群
肠黏膜菌群
多样性
相对丰度
mice
Dendrobium officinale polysaccharides
lumen-associated microbiota
mucosa-associated microbiota
diversity
relative abundance
