摘要
目的检测传染性单核细胞增多症(IM)患者及非本病的发热患者外周血T淋巴细胞亚群,探讨EB病毒感染导致的传染性单核细胞增多症的免疫反应机制及与非本病发热患者的鉴别诊断价值,同时通过检测患者血液中的EBV-DNA载量加以确证。方法选取确诊为传染性单核细胞增多症的患者30例(IM组),非传染性单核细胞增多症的发热患者30例(对照组)。应用流式细胞技术检测患者外周血中淋巴细胞亚群,同时应用荧光定量PCR技术检测血清EBV-DNA载量,采用卡方检验及Mann-Whitney检验进行组间差异分析。结果IM组与对照组患者T淋巴细胞亚群相比,CD8^(+)T淋巴细胞所占比例显著升高(Z=1.776,P<0.001),CD4^(+)T淋巴细胞所占比例降低(F=4.008,P<0.050),同时CD4^(+)/CD8^(+)比例明显下降(Z=6.653,P<0.001)。IM组EBV-DNA病毒载量显著高于对照组,差异具有统计学意义(P<0.050)。结论传染性单核细胞增多症患者外周血T淋巴细胞亚群主要以CD4^(+)T淋巴细胞及CD8^(+)T淋巴细胞的变化为主,T淋巴细胞亚群结合血清EBV-DNA病毒载量的联合检测对IM及非本病的发热患者具有鉴别诊断价值。
Objective To detect T lymphocyte subsets and EBV-DNA load in peripheral blood,and investigate their diagnostic significance for patients with infectious mononucleosis(IM)and non-IM patients with fever.Methods Thirty patients diagnosed as IM(IM group)and 30 non-IM patients with fever(control group)were enrolled.The lymphocyte subsets in peripheral blood and EBV DNA load were detected respectively by using flow cytometry and fluorescence quantitative polymerase chain reaction(PCR).Differences between groups were analyzed using chi-square test and Mann-Whitney test.Results Compared with the control group,the proportion of CD8^(+)T lymphocytes increased significantly(Z=1.776,P<0.001),that of CD4^(+)T lymphocytes reduced obviously(F=4.008,P<0.05),and that of CD4^(+)/CD8^(+)decreased significantly(Z=6.653,P<0.001)in the IM group.The viral load of EBV-DNA in IM group was significantly higher than that in control group(P<0.05).Conclusion CD4^(+)and CD8^(+)T lymphocytes were the main subsets of peripheral blood T lymphocytes in patients with infectious mononucleosis,and atypical lymphocytes increased.Combined determination of T lymphocyte subsets and serum EBV-DNA viral load has differential diagnostic value for both IM patients and non-IM patients with fever.
作者
袁晓燕
葛丽娜
赵家兴
罗红
YUAN Xiao-yan;GE Li-na;ZHAO Jia-xing;LUO Hong(Laboratory,Weihai Municipal Hospital,Weihai,Shandong 264200,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2021年第6期679-682,共4页
Chinese Journal of Microecology
基金
辽宁省自然科学基金(20180550460)。
