摘要
为探究在低碳源条件下,酸性硒、镉废水生物同步处理的可行性,采用厌氧颗粒污泥间歇反应器(SBR)对含硒(7.9 mg·L^(-1))、镉(11.2 mg·L^(-1))酸性废水进行了处理。结果表明:在进水COD为100 mg·L^(-1)的第I阶段(1~40周期),镉平均去除率为(96.67±2.70)%,在第I阶段的前16个周期,总硒和四价硒的平均去除率分别达到(97.07±3.17)%和(98.86±1.84)%,自第17周期起,总硒和四价硒的去除率逐渐下降至第40周期的(54.47±0.62)%和(57.66±2.24)%;当进水COD为50 mg·L^(-1)的第II阶段(41~100周期),对废水中镉、硒的去除效果均不理想。扫描电镜-X射线能谱(SEM-EDS)和X射线光电子能谱(XPS)分析表征结果表明,厌氧颗粒污泥对Se(Ⅳ)、Cd(Ⅱ)的去除机理包括生物还原和吸附,产物中出现Se(0)和硒化镉(CdSe)纳米颗粒。细菌群落分析结果表明,在细菌门水平上,Proteobacteria(34.11%)、Synergistota(31.16%)和Firmicutes(12.48%)为优势菌;在细菌科水平上,具有较强硒、镉去除能力的Burkholderiaceae(34.07%)、Comamonadaceae(10.84%)和Pseudomonadaceae(6.01%)在厌氧颗粒污泥中占据优势地位。古菌群落结构分析结果表明,具有较强重金属耐受能力的Methanosaeta和Methanobacterium古菌相对丰度最高,分别为51.00%和46.98%。以上研究结果可为含硒、镉低碳源酸性废水处理及其微生物特性的研究提供参考。
In order to explore the feasibility of biological treatment of acidic selenium and cadmium wastewater synchronously with limited carbon source,sequencing batch reactor(SBR)inoculated with anaerobic granular sludge was used to treat acidic wastewater containing selenium(7.9 mg·L^(-1))or cadmium(11.2 mg·L^(-1)).The results showed that at the stage Ⅰ(1~40 cycles)with the influent COD of 100 mg·L^(-1),the average removal efficiency of cadmium was(96.67±2.70)%.While in the first 16 cycles of stage Ⅰ,the average removal efficiencies of total Se and Se(Ⅳ)were(97.07±3.17)% and(98.86±1.84)%,respectively.From the 17 th cycle,the removal efficiencies of total Se and Se(Ⅳ)decreased gradually to(54.47±0.62)%and(57.66±2.24)%at the40 th cycle,respectively.At the stage Ⅱ(41~100 cycles)with the influent COD of 50 mg·L^(-1),the removal efficiencies of cadmium and selenium in wastewater were low.Scanning electron microscopy coupled with energy dispersive X-ray spectroscopy(SEM-EDS)and X-ray photoelectron spectrometer(XPS)results showed that the mechanisms of Se(Ⅳ) and Cd(Ⅱ) removal by anaerobic granular sludge included biological reduction and adsorption,and Se(0)and CdSe nanoparticles occurred in the biomass.The results of bacterial community analysis showed that Proteobacteria(34.11%),Synergistota(31.16%)and Firmicutes(12.48%)were the dominant bacteria at the phylum level.Burkholderiae(34.07%),Comamonadaceae(10.84%)and Pseudomonadaceae(6.01%)with stronger ability to remove selenium and cadmium,were dominant in the anaerobic granular sludge at the family level.The analysis of the archaeal community structure showed that Methanosaeta and Methanobacterium with strong heavy metal tolerant had the highest relative abundances of 51.00% and 46.98%,respectively.This study provides the reference for treating acidic wastewater containing selenium or cadmium with limited carbon source,as well as the corresponding microbial community characteristic analysis.
作者
曾涛涛
胡青
张晓玲
王亮钦
蔡萍莉
张宇
刘迎九
ZENG Taotao;HU Qing;ZHANG Xiaoling;WANG Liangqin;CAI Pingli;ZHANG Yu;LIU Yingjiu(Hunan Provincial Key Laboratory of Pollution Control and Resources Technology,University of South China,Hengyang 421001,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2021年第6期2080-2090,共11页
Chinese Journal of Environmental Engineering
基金
湖南省自然科学青年基金项目(2019JJ50507)
中科院环境生物技术重点实验室开放课题(kf2018001)
2020年度湖南省大学生创新实验项目(湘教通[2020]191号-2797)
2018年南华大学大学生创新实验项目(2018XJXZ002)。
关键词
硒
镉
厌氧颗粒污泥
微生物群落
selenium
cadmium
anaerobic granular sludge
microbial community
