摘要
废纸制浆废水厌氧处理时会发生严重厌氧颗粒污泥钙化问题,而造纸厂蔗渣喷淋废水厌氧处理时则极少出现颗粒污泥钙化现象,值得我们进行深入研究。取正常运行条件下蔗渣喷淋废水厌氧处理颗粒污泥AS1和废纸废水厌氧处理的颗粒污泥AS2进行表面结构、元素组成及微生物结构分析,为揭示颗粒污泥钙化原因提供理论和数据支持。通过电感耦合等离子质谱仪(ICP-MS)分析污泥的重金属成分、利用红外光谱(FITR)分析污泥表面官能团种类、采用能谱仪(EDS)分析污泥表面的元素组成、并使用Illumina Hi Seq 2500对细菌V3和V4区进行高通量测序,分析了污泥微生物的多样性及菌群的相对丰度。结果表明,两个样品的重金属含量、表面元素分布、表面官能团分布均存在较大差别。AS1中的微生物主要由Bactericides(25.37%)、Proteobacteria(20.19%)、Hyd24-12(14.43%)、Chloroflexi(10.58%)和Firmicutes(8.91%)五个菌门组成,AS2中的微生物主要由Bacteroidetes(20.44%)、Chloroflexi(19.47%)、Proteobacteria(19.32%)、Firmicutes(6.15%)、Spirochaetae(4.81%)、Actinobacteria(4.39%)和Lentisphaerae(4.32%)七个菌门组成,而且细菌种类比较丰富,且细菌数量分布与其处理底物相适应。另外,在AS1和AS2中都检测到Methanobacterium和Methanosaeta两类产甲烷古菌,单独在AS2中检测到了第七产甲烷古菌目代表菌Methanomassiliicoccus。AS2中产甲烷菌的相对丰度较高,约占50%;而AS1仅占10%。
Dominant bacterial community structures in the anaerobic granular sludge plays key roles during the treatment of papermaking wastewater in internal circulation anaerobic reactor （IC）. Surface structure, element composition and microbial structure in anaerobic granular sludge samples from two different sources AS1 （bagasse pulping wastewater） and AS2 （wastepaper recycling wastewater） were studied. The results showed that there were signifi- cant differences between them. The microbes in AS1 were mainly composed of Bacteroidetes （25.37%）, Pro-teobacteria （20. 19% ）, Hyd24 - 12 （ 14. 43% ）, Chloroflexi （ 10.58% ） and Firmicutes （8.91%）, in AS2 were mainly composed of Bacteroidetes （ 20.44% ）, Chloroflexi （ 19.47% ）, Proteobacteria （ 19.32% ）, Firmicutes （6. 15% ）, Spirochaetae （4. 81% ）, Actinobacteria （4.39%） and Lentisphaerae （4. 32% ）. In addition, both Methanobacterium and Methanosaeta were found as methanogenic archaea, but particularly finding the Methanomas-siliicoccus in AS2, and the total relative abundance of methanogens in AS2 was higher than that in AS1, especially the Methanosaeta.
出处
《造纸科学与技术》
2017年第3期72-78,共7页
Paper Science & Technology
基金
国家自然科学基金(No.31660182)
广西青年自然科学基金(No.2015GXNSFBA139042)
广西清洁化制浆造纸与污染控制重点实验室基金项目(ZR201607)
关键词
造纸废水
厌氧颗粒污泥
高通量测序
微生物群落
microbial community
high - throughput sequencing
papermaking wastewater
anaerobic granular sludge
