摘要
Dear Editor,The current pandemic of beta-coronavirus(SARS-CoV-2)has exerted devastating influence on almost all countries,resulting in the disease named COVID-19.1 Coronavirus possesses the largest RNA genome among all the RNA viruses.Its genome encodes about 29 proteins(Supplementary Fig.S1).The subcellular distributions of the viral proteins have yet been reported for SARS-CoV-2.It is important to investigate the viral proteins’locations in cells because the subcellular distribution information not only helps us in understanding how viruses interact with the host cells but also provides clues in fighting against the viral infection.Therefore,we cloned all the genes of SARS-CoV-2 into vectors for expression in mammalian cells and used immunofluorescent assay(IFA)to examine the viral proteins’subcellular location.Except for the NSP11 that is only 14 aa long,we expressed all other 28 viral proteins in HEp-2 or Caco-2 cells and found a diversity of protein distribution in cells,suggesting a complicated interaction of SARSCoV-2 with host cells to achieve a successful infection.
基金
supported by an NIH/NIAID SC1AI112785(Q.T.)
an NIH/DE R01DE028583-01(subaward to Q.T.)
National Institute on Minority Health and Health Disparities of the National Institutes of Health under Award Number G12MD007597(Q.T.)
by grants from COVID-19 emergency tackling research project of Shandong University(Grant No.2020XGB03 to P.-H.W).
