摘要
在原有实验基础上对铬革屑水解工艺参数进行了进一步优化。增加的水洗预处理步骤可以显著降低铬革屑中40%的灰分含量。单因素优化实验结果显示,较佳的工艺参数为氧化钙脱铬温度65℃、氧化钙用量10%、碱处理液比10、氧化钙处理时间30 min、酶水解温度60℃、酶用量0.8%、酶水解时间1.0 h。正交优化实验结果表明,最佳工艺条件为氧化钙脱铬温度68℃、氧化钙用量10%、液比9、碱处理时间25 min、酶水解温度57℃、酶用0.7%、酶水解时间1.0 h,调节水解液pH10.5,静止30min,过滤,弃渣留液。最优工艺方案的水解率为76.79%,铬含量为44.30 mg/kg。最优工艺下胶原蛋白提取率为84.86%,灰分含量8.14%。
This paper further optimized the hydrolysis process parameters of chrome shavings based on the original experiment.The additional water washing pretreatment step can significantly reduce the ash content in chrome shavings by 40%.The results of single factor optimization experiments showed that the best process parameters are calcium oxide dechrome temperature 65℃,calcium oxide dosage 10%,alkali treatment solution ratio 10,the treatment time of calcium oxide 30min,the hydrolysis temperature of enzyme 60 h,the dosage of enzyme 0.8%,and the hydrolysis time of enzyme 1.0h.Orthogonal optimization experiment results showed that the optimal process conditions were as follows:calcium oxide dechromation temperature 68℃,calcium oxide dosage 10%,liquid ratio 9,alkali treatment time 25min,enzymatic hydrolysis temperature 57℃,enzyme dosage 0.7%,enzyme hydrolysis time 1.0 h,hydrolysate pH 10.5,static for 30 min,filtration,discarding residue and leaving liquid.The hydrolysis rate of the optimal process was 76.79%,and the chromium content was 44.30 mg/kg.Under the optimal process,the extraction rate of collagen is 84.86%and the ash content is 8.14%.
作者
王文靖
卢欣雨
张晓伟
刘彦
WANG Wenjing;LU Xinyu;ZHANG Xiaowei;LIU Yan(Department of Biomass and Leather Engineering,Sichuan University,Chengdu 610065,China;National Engineering Research Center of Clean Technology in Leather Indusrty,Sichuan University,Chengdu 610065,China)
出处
《皮革科学与工程》
CAS
北大核心
2021年第4期40-45,共6页
Leather Science and Engineering
基金
四川省科技厅项目资助(2019YFG0273)。
关键词
铬革屑
酶水解
碱-酶法
工艺优化:正交试验
chrome shavings
enzymatic hydrolysis
alkaline-enzymatic method
process optimization:orthogonal test
