摘要
目的探讨N-乙酰半胱氨酸(N-acetylcysteine,NAC)改善亚急性暴露于大气细颗粒物(particulate matter,PM)2.5致大鼠睾丸损伤的作用。方法30只Wistar大鼠随机分为3组,分别为生理盐水对照组、PM2.5暴露组、PM2.5+N-乙酰半胱氨酸组。通过气管内滴注的方法进行染毒,每次剂量为5.0 mg/kg,每周染毒一次,共8周;NAC组在PM2.5染毒的基础上,通过灌胃方式连续给予150 mg·kg^(-1)·d^(-1) NAC;生理盐水对照组通过气管内滴注同体积生理盐水(1 mL/kg)作为对照。造模后HE染色制作睾丸组织病理切片,TUNEL法检测睾丸凋亡情况,分光光度计法检测睾丸组织超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)、和丙二醛(malondialdehyde,MDA)指标,酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)法测定睾丸白细胞介素10(interleukin-10,IL-10)及睾酮水平。结果PM2.5暴露组和PM2.5+NAC组大鼠血清睾酮水平及睾丸组织GSH、SOD、IL-10水平均低于盐水对照组,睾丸组织MDA水平升高,PM2.5+NAC组睾酮水平及睾丸组织GSH、SOD、IL-10水平高于PM2.5组,睾丸组织MDA水平下降(P<0.05)。在PM2.5暴露组中,曲精小管中多个细胞核表现出明显的深棕色染色,TUNEL阳性细胞的计数明显高于盐水对照组和PM2.5+NAC组(P<0.05);而PM2.5+NAC组凋亡细胞数明显低于PM2.5组,但高于盐水对照组(P<0.05)。PM2.5暴露组和PM2.5+NAC组睾丸细胞凋亡指数高于盐水对照组,PM2.5+NAC组低于PM2.5暴露组(P<0.05)。结论PM2.5亚急性暴露可以造成大鼠睾丸组织病理损伤,诱导睾丸细胞凋亡,引起氧化炎症指标改变,而NAC作为一种抗氧化剂,可以明显改善上述损伤作用,对PM2.5致睾丸毒性作用具有拮抗作用。
Objective To investigate the effect of N-acetylcysteine(NAC)on ameliorating testicular injury of rats caused by subacute exposure to atmospheric fine particulate matter(PM2.5).Methods A total of 30 Wistar rats were randomly divided into three groups:saline control group,PM2.5 exposure group and PM2.5+NAC group.The rats in PM2.5 exposure group were exposed to a dose of 5.0 mg/kg PM2.5 suspension by intratracheal instillation,once a week for 8 weeks.Rats in the PM2.5+NAC groups were given 150 mg·kg^(-1)·d^(-1) NAC by intragastric gavage continuously on the basis of PM2.5 exposure.Saline control group was given the same volume of saline(1 mL/kg).After modeling,the pathological sections of testicular tissue were prepared by hematoxylin-eosin staining(HE).Terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)method was used to detect testicular apoptosis in rats.Superoxide dismutase(SOD),glutathione(GSH)and malondialdehyde(MDA)indicators were measured using spectrophotometer method.The levels of interleukin-10(IL-10)and testosterone were measured by enzyme-linked immunosorbent assay(ELISA)method.Results Serum testosterone levels and testicular tissue GSH,SOD,IL-10 levels of rats in the PM2.5 exposure group and PM2.5+NAC group were lower than those in the saline control group,while MDA levels in the testicular tissue were increased;The testosterone levels and testicular tissue GSH,SOD,IL-10 levels were higher in the PM2.5+NAC group than in PM2.5 exposure group,while MDA level in testicular tissue was decreased(P<0.05).In the PM2.5 exposure group,multiple nuclei in the seminiferous tubules showed obvious dark brown staining,and the TUNEL positive cell count was significantly higher than that of the saline control group and the PM2.5+NAC group(P<0.05).The number of apoptotic cells in the PM2.5+NAC group was significantly lower than that of the PM2.5 control group,but higher than that of the saline control group(P<0.05).The apoptosis index of testicular cells in the PM2.5 exposure group and PM2.5+NAC group
作者
田硕
张宁
牛姝
李珅
苏力
平芬
TIAN Shuo;ZHANG Ning;NIU Shu;LI Shen;SU Li;PING Fen(Department of Urinary Surgery, the First Hospital of Shijiazhuang City,Hebei Province, Shijiazhuang 050011, China;Department of Geratology, Hebei General Hospital, Shijiazhuang 050051, China;Department of Endocrinology, the First Hospital of Shijiazhuang City, Hebei Province, Shijiazhuang 050011, China)
出处
《河北医科大学学报》
CAS
2021年第7期754-758,778,共6页
Journal of Hebei Medical University
基金
河北省医学科学研究重点课题(20180997)。
关键词
细颗粒物
N-乙酰半胱氨酸
氧化应激
炎症
凋亡
particulate matter(PM)2.5
N-acetylcysteine
oxidativestress
inflammation
apoptosis
