摘要
目的探讨hMSH3rs1428030基因多态性和基因-环境交互作用与乙肝相关性肝癌(HBV-HCC)易感性的关系。方法采用以医院为基础的病例对照研究,收集三甲综合医院和专科医院的HBV-HCC(病例组)和乙肝(对照组)患者各198例。通过调查表以面对面的方式收集研究对象的资料,应用SnapShot小测序对位点进行基因型的检测。采用Excel 2007构建数据库,SPSS 17.0和MDR 2.0进行数据分析,t检验和χ2检验分析单因素影响,Logistic回归进行多因素分析,多因子降维(MDR)法分析交互作用影响。结果病例组和对照组年龄(t=-11.51)、HBV感染时长(χ2=44.54)、饮酒史(22χ=21.04)和吸烟史(χ=18.24)差异有统计学意义,P<0.05;单因素和多因素分析均显示,病例组和对照组hMSH3rs1428030位点AA、AG和GG基因型频率分布差异有统计学意义;相乘模型表明,HBV感染时长与hMSH3rs1428030基因多态性存在基于相乘模型的交互作用(OR=3.29);多因子降维法显示,hMSH3rs1428030和HBV感染时长、吸烟3因子模型差异有统计学意义(P=0.016),可以认为hMSH3rs1428030、HBV感染时长和吸烟的交互作用与HBV-HCC易感性相关,交互效应值为5.05(95%CI:1.31~19.50)。结论 hMSH3rs1428030基因多态性可能为HBV-HCC发病的遗传易感因素,且与HBV感染时长和吸烟具有协同致癌作用。
Objective To investigate the relationship between hMSH3 rs1428030 gene polymorphism and gene-environment interaction and the susceptibility to hepatocellular carcinoma associated with Hepatitis B(HBV-HCC).Methods A hospital-based case-control study was conducted to collect 198 patients with HBV-HCC and hepatitis B patients in the top three general hospitals and specialist hospitals.The data of the subjects were collected in a face-to-face manner through questionnaires.The genomic detection of the locus was performed using SnapShot small sequencing.The database was built by Excel 2007,SPSS 17.0 and MDR 2.0 statistical software were used for data analysis;t test and2 χtest analysis for single factor effects,Logistic regression for multivariate analysis,and Logistic regression and multi-factor dimensionality reduction(MDR)method for analysis of interaction effects.Results The age(t=-11.51),duration of HBV infection(χ2=44.54),history of drinking(χ2=21.04),and smoking history(χ2=18.24)were statistically significant (all P<0.05);A univariate and multivariate analysis showed that the frequency distribution of AA,AG and GG genotypes in the hMSH3 rs1428030 locus in the case group and the control group was statistically significant.The multiplicative model showed that there was a multiplicative model-based interaction between HBV infection duration and hMSH3 rs1428030 polymorphism(OR=3.29).Multi-factor dimensionality reduction MDR showed that hMSH3 rs1428030 and HBV infection duration,smoking 3-factor model were statistically significant(P=0.016),the interaction between hMSH3 rs1428030,HBV infection duration and smoking was associated with HBV-HCC susceptibility,with an interaction value of 5.05(95%CI:1.31-19.50).Conclusion hMSH3 rs1428030 gene polymorphism may be a genetic susceptibility factor for the occurrence of HBV-HCC,and has synergistic carcinogenic effects with the duration of HBV infection and smoking.
作者
刘英
韩亚男
佟松播
刘晓刚
LIU Ying;HAN Ya-nan;TONG Song-bo;LIU Xiao-gang(Department of Science Education,North China University of Science and Technology Affiliated Hospital,Tangshan 063000,China;Department of Human Resouce,Chaoyang Central Hospital,Chaoyang 122000,China;School of Public Health,North China University of Science and Technology,Tangshan 063000,China;The Second Department of Hepatobiliary Surgery,Tangshan Workers Hospital,Tangshan 063000,China)
出处
《中华肿瘤防治杂志》
CAS
北大核心
2021年第12期909-913,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
河北省自然基金重大项目(H2016206576)。
关键词
hMSH3
单核苷酸多态性
乙肝相关性肝癌
交互作用
hMSH3
single nucleotide polymorphism
hepatitis B-related hepatocellular carcinoma
interaction
